| BackgroundCoronary heart disease(CHD)is coronary artery stenosis or blockage caused by atherosclerotic lesions,and then causes heart disease mainly manifested by myocardial ischemia,hypoxia,and necrosis.The risk factors include lipid metabolism,gene polymorphism,etc.Apolipoprotein(Apo)can be involved in the occurrence and development of hyperlipidemia,CHD and cardiovascular and cerebrovascular diseases by influencing the metabolism and utilization of blood lipids.With the deepening of the research on the expression regulation and polymorphism of Apo coding genes in recent years,It was found that the polymorphism of apolipoprotein AII-265T/C(Apo AII-265T/C)gene locus can reduce the susceptibility to atherosclerosis,but its specific regulation effect on lipid metabolism remains unclear..Based on this,this study took patients with CHD as the research object to explore the correlation between Apo AII-265T/C gene polymorphism and lipid metabolism in patients with CHD.ObjectiveTo investigate the correlation between Apo AII-265T/C polymorphism and lipid metabolism in patients with CHD.MethodsNinety-five patients with CHD who visited our hospital from April 2022 to October 2022 were defined as the CHD group,and 100 healthy volunteers who underwent physical examination in our hospital during the same period were defined as the control group,the Apo AII-265T/C gene polymorphisms were detected by Polymerase chain reaction-restriction fragment leng(PCR-RFLP);serum total cholesterol(TC),triglyceride(TG)and high density lipoprotein cholesterol(HDL-CHOLESTEROL)were measured by automatic biochemical analyzer and matching kit.HDL-C),low-density lipoprotein cholesterol(LDL-C),apolipoprotein AI(Apo AI),apolipoprotein AII(apolipoprotein AII,Apo AII and apolipoprotein B,Spearman correlation analysis was used to analyze the correlation between Apo AII-265T/C polymorphism and lipid metabolism indexes in CHD patients.All CHD patients received coronary angiography,and the degree of coronary artery stenosis was evaluated according to Gensini scoring system.Clinical data of CHD patients were collected,and the risk factors that might affect CHD were screened by univariate analysis,and whether Apo AII-265T/C gene polymorphism was a risk factor for CHD was determined by Logistic multivariate regression analysis.Results(1)The number of patients aged > 60 years old,smoking cases,hypertension cases and hyperlipidemia cases in CHD group was higher than that in control group,with statistical significance(P<0.05);(2)The frequencies of CC genotype and C allele in CHD group were higher than those in control group,and the difference between groups was statistically significant(P<0.05),indicating that CC genotype and C allele might be involved in the occurrence of CHD;the genotype frequency distribution of the study population was consistent with Hardy-Weinberg law(χ2=0.005,P=0.924),suggesting that the study population was representative to a certain extent;(3)CHD patients with TC,TG,LDL-C level were higher than control group,HDL-C,Apo A I,Apo AII,Apo B levels are lower than the control group,the differences between groups were statistically significant(P<0.05),namely,lipid metabolism,apolipoprotein may be associated with the occurrence of CHD;(4)Spearman correlation analysis showed that the CC genotype was positively correlated with TC,TG and LDL-C in CHD patients(r=0.416,0.313,0.495,P<0.05),and negatively correlated with HDL-C(r=-0.580,P<0.05),that is,the risk of lipid metabolism disorder in CHD patients carrying CC genotype was higher than that in CHD patients without CC genotype;(5)Logistic regression analysis showed that age > 60 years old,smoking,hypertension,hyperlipidemia,LDL-C,Apo AII,Apo B,CC genotype and C allele were independent risk factors for CHD,and Apo AI was independent protective factor for CHD.ConclusionAge > 60 years old,smoking,hypertension,hyperlipidemia,LDL-C,Apo A Ⅰ,Apo AII,Apo B,CC genotype and C allele were independent risk factors for CHD,and Apo AI was independent protective factor for CHD.The CC genotype and C allele frequency of Apo AII-265T/C were correlated with lipid metabolism in CHD patients. |
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