Study On The Level Of Brain-gut Peptide In Patients With Gastroesophageal Reflux Disease With Anxiety And Depression Of Different TCM Syndromes

Objective: By detecting the serum levels of brain gut peptide in patients with different types of gastroesophageal reflux disease and anxiety and depression,we aim to explore the distribution of brain gut peptide levels in patients with different types of traditional Chinese medicine gastroesophageal reflux disease and anxiety and depression.This will provide more objective basis for the traditional Chinese medicine syndrome differentiation and treatment of gastroesophageal reflux disease and anxiety and depression,thereby better guiding the traditional Chinese medicine syndrome differentiation and treatment of this disease.Methods: 85 GERD patients diagnosed with anxiety and depression were included in the outpatient department and ward of the Spleen and Stomach Department of the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine from August 2021 to August 2022.Patients diagnosed with gastroesophageal reflux disease completed the Self Rating Anxiety Scale(SAS)and Self Rating Depression Scale(SDS)scores.According to the consensus on the diagnosis and treatment of gastroesophageal reflux disease in traditional Chinese medicine,the syndrome diagnosis criteria are divided into six types: liver stomach stagnation heat syndrome,gallbladder heat invading stomach syndrome,middle deficiency qi inversion syndrome,qi stagnation phlegm obstruction syndrome,blood stasis blocking collaterals syndrome,and spleen stomach dampness heat syndrome.Enzyme linked immunosorbent assay(ELISA)was used to detect the serum levels of brain gut peptides Ghrelin,Leptin,CGRP,NPY,and 5-HT in patients with anxiety and depression in GERD.Using SPSS 23.0 statistical software for data analysis,the measurement data is represented by mean ±standard deviation(c ± s);The measurement data conforming to normal distribution shall be subject to t test,and the measurement data not conforming to normal distribution shall be subject to non parametric test.A P-value of<0.05 indicates that the results are statistically significant.By analyzing and exploring the distribution of brain gut peptide levels in patients with different traditional Chinese medicine syndrome types of gastroesophageal reflux disease accompanied by anxiety and depression.Results:(1)The SAS and SDS scores of GERD patients with anxiety and depression showed statistically significant differences among traditional Chinese medicine syndrome types(P<0.05),with the highest scores in liver stomach stagnation heat syndrome and gallbladder heat invasion stomach syndrome.(2)There was no statistically significant difference in the levels of leptin and CGRP among different traditional Chinese medicine syndrome types in GERD patients with anxiety and depression(P>0.05).(3)The levels of Ghrelin,NPY,and 5-HT showed statistically significant differences among different traditional Chinese medicine syndrome types in GERD patients with anxiety and depression(p<0.05).There was a statistically significant difference in the serum levels of Ghrelin between the liver stomach stagnation heat syndrome and the middle deficiency qi inversion syndrome,as well as between the gallbladder heat invasion stomach syndrome and the middle deficiency qi inversion syndrome(p<0.05).The serum levels of NPY showed statistical significance(p<0.05)between liver stomach stagnation heat syndrome and qi stagnation phlegm obstruction syndrome,liver stomach stagnation heat syndrome and middle deficiency qi inversion syndrome,gallbladder heat invasion stomach syndrome and qi stagnation phlegm obstruction syndrome.The serum levels of 5-HT showed statistical significance(p<0.05)between the liver stomach stagnation heat syndrome and the middle deficiency qi inversion syndrome,the liver stomach stagnation heat syndrome and the qi stagnation phlegm obstruction syndrome,the liver stomach stagnation heat syndrome and the stasis blood obstruction collaterals syndrome,the liver stomach stagnation heat syndrome-spleen stomach damp heat syndrome,the gallbladder heat invasion stomach syndrome and the middle deficiency qi inversion syndrome,the gallbladder heat invasion stomach syndrome and the qi stagnation phlegm obstruction syndrome,the gallbladder heat invasion stomach syndrome and the stasis blood obstruction collaterals syndrome,and the gallbladder heat invasion stomach syndrome-spleen stomach damp heat syndrome.Conclusion(s):(1)The degree of anxiety and depression in patients with liver stomach stagnation heat syndrome and gallbladder heat invading stomach syndrome GERD accompanied by anxiety and depression is even greater.(2)There is no significant correlation between the levels of leptin and CGRP and the traditional Chinese medicine syndrome types of GERD with anxiety and depression.(3)The levels of Ghrelin,NPY,and 5-HT are related to the traditional Chinese medicine syndrome types of GERD accompanied by anxiety and depression.Ghrelin has the lowest level in the syndrome of middle deficiency qi inversion,while NPY has a lower level in the syndrome of middle deficiency qi inversion and phlegm qi depression.The level of5-HT is lower in the syndrome of liver stomach stagnation heat and gallbladder heat invading stomach.