Sea Cucumber Ovum Extract Improves Premature Ovarian Failure Pharmacological Activity And Mechanism Research

Sea cucumber ovum is the gonadal tissue of sea cucumber.It has long been neglected as waste in the processing of sea cucumber industry.In recent years,research on sea cucumber ovum shows that it has a wide range of potential for development and application in functional food and medical treatment.back into the public eye.At present,the application of sea cucumber ovum is mainly based on direct consumption by a small number of people,and most of the materials are still discarded.There is still a long way to achieve the high value-added development and utilization of sea cucumber ovum.In this paper,sea cucumber ovum powder was used as raw material,and different components of sea cucumber ovum were prepared by various chemical separation methods.Further in-depth verification of the component’s animal function level and animal molecular level in mouse with premature ovarian failure to evaluate related functional activities and explore molecular mechanisms.The specific research methods and experimental results are as follows:(1)Separation,Preparation and Characterization of Different Components of Sea Cucumber ovum: The ethanol extract component of Sea Cucumber ovum was obtained after three consecutive extractions of Sea Cucumber pollen powder(with a yield of 19.7%).Subsequently,after three consecutive extractions of the ethanol extract from sea cucumber ovum,the aqueous phase components of the ethanol extract from sea cucumber ovum(with a yield of 12.57%)and the n-hexane phase components of the sea cucumber ovum extract(with a yield of 10.15%)were obtained.The substances contained in the four components were preliminarily identified.(2)Preliminary pharmacological study of different sea cucumber ovum extracts on mice with premature ovarian failure:Preliminary pharmacodynamic screening was conducted using ovarian organ index as an indicator by setting up two administration methods: treatment group and prevention group.The results showed that the treatment group had better effects than the prevention group,with aqueous substances having the best effect.(3)The pharmacological effects of ethanol extract from sea cucumber sea cucumber ovum on mice with premature ovarian failure were studied.The results showed that there was no significant difference in the body weight of each group of experimental animals.The administration of water phase substances significantly increased the ovarian organ index of mice with premature ovarian failure,significantly increased the serum levels of anti-Mullerian hormone(AMH)and estradiol(E2),and significantly reduced the levels of follicle stimulating hormone(FSH)in mice with premature ovarian failure,Improved estrous cycle disorder and egg development.(4)Exploration of the Mechanism of Ethanol Extracts from Sea cucumber ovum in Improving Premature Ovarian Failure in Mice: sea cucumber ovum extract can inhibit the activation of the PI3K/AKT/m TOR signaling pathway in ovarian tissue to enhance ovarian reserve,alleviate apoptosis in ovarian tissue to protect ovarian function,and improve the expression of estrogen receptors.In summary,the development of this experiment confirmed the pharmacological effect of sea cucumber ovum extract on improving premature ovarian failure and studied its mechanism of action.