Pharmaceutical Study On Seabuckthorn Haematococcus Pluvialis Capsules

Oxidative stress damage induced by increased free radicals has been shown to cause damage to macromolecules such as sugars,proteins lipids,and DNA,which has a negative impact on the biological state of the human body,thereby promoting the incidence of various diseases and reducing life expectancy.With the growth of people’s age and the influence of exogenous factors,the endogenous antioxidant system cannot effectively offset the large amount of free radicals,and the problem of oxidative stress becomes more acute.Synthetic antioxidants have been proved to have certain toxicity and carcinogenic effects.Therefore,finding natural antioxidants that can scavenge free radicals in the body has become the development trend of the pharmaceutical and health food industries.Based on this,according to the etiology and pathogenesis of oxidative stress and the physical characteristics of middle-aged and elderly people,this topic combines Seabuckthorn and Haematococcus Pluvialis to have the effects of tonifying qi,invigorating spleen and kidney,soothing liver and relieving depression,promoting blood circulation and regulating qi,regulating yin and yang,and protecting the body from oxidative damage.In this paper,the extraction process,preparation on process,quality standard,safety,antioxidant function,and stability of Seabuckthorn Haematococcus Pluvialis Capsules were systematically studied.Because the active ingredient of sea buckthorn is flavonoid glycosides,which is suitable for water extraction,and can use alcohol precipitation to remove tannins,amino acids,proteins and other components that have no obvious effect,and enrich the content of flavonoid glycosides,it is determined to carry out water extraction and alcohol precipitation of sea buckthorn.The amount of Haematococcus pluvialis used is small,and the astaxanthin components contained are easily oxidized during the extraction and purification process,so the whole powder of Haematococcus pluvialis is used as medicine.The content of isorhamnetin in seabuckthorn and the yield of dry extract were used as evaluation indexes to analyze the two response surface models of water extraction and alcohol precipitation,and the process was verified to obtain the best extraction process.According to the daily dosage,Seabuckthorn dry extract powder and Haematococcus pluvialis powder were mixed in proportion.However,due to its poor fluidity and extremely hygroscopicity,it needs to be improved by adding excipients and granulation.After a variety of fillers were uniformly mixed with the raw material powder,the corresponding moisture absorption rates at different times were measured,and the moisture absorption-time curve was drawn.By observing the appearance and analyzing the fitted equation,the final filler and drug-assisted ratio were determined.The wetting agent was screened by granulation and particle forming rate.Because the free astaxanthin in Haematococcus pluvialis is easy to oxidize,in order to ensure the stability of the preparation,the zein solution was sprayed on the surface of the particles to achieve the effect of oxygen and moisture isolation,so that the content of astaxanthin remained stable and not degraded.Finally,the angle of repose,bulk density and relative critical humidity of the formed particles were investigated to determine the best preparation process.Establish quality standards.Firstly,the conventional items such as appearance,moisture,loading difference and disintegration time limit were examined,and the qualitative identification of Seabuckthorn and Haematococcus pluvialis powder was carried out by thin layer analysis.The content of total astaxanthin was determined by UV method,and the content of isorhamnetin and all-trans astaxanthin was determined by HPLC method.The content standard of these three components was set:isorhamnetin≥20mg/100g;total astaxanthin≥0.5g/100g;all-trans astaxanthin≥0.22g/100 g.According to the national health food safety toxicology test and evaluation of technical specifications,acute toxicity test,genetic toxicity test and 28-day toxicity test were carried out on Seabuckthorn Haematococcus pluvialis capsules.There was no obvious toxic reaction,which was actually non-toxic and safe to eat.According to the antioxidant function evaluation method in the document [2012]107 of the State Food and Drug Administration,the antioxidant function was evaluated with antioxidant enzymes,protein oxidation products,antioxidant substances and lipid oxidation products as indicators.The results of the four indicators were all positive,and the sample could be identified as having antioxidant function.Preliminary stability check.The results showed that each index was stable in accelerated test and the long-term test up to 9 months,which provided a scientific basis for the formulation of validity period,storage and transportation conditions of Seabuckthorn Haematococcus pluvialis capsules.