Treatment Of Alopecia Areata With Hair Follicle Mesenchymal Stem Cells And Its Mechanism

Abstract:Background:Alopecia areata(AA)is a common autoimmune hair loss disease with increasing incidence.Corticosteroids are the most widely used for hair loss treatment;however,long?term usage of hormonal drugs is associated with various side effects.Mesenchymal stem cells(MSC)therapy has been studied extensively to cure autoimmune diseases without affecting immunity against diseases.Methods:Hair follicle?derived MSC(HF?MSC)were harvested from the waste material of hair transplants,isolated,expanded and identified by flow cytometry,immunofluorescence,differentiation capacity.The therapeutic effect of HF?MSC for AA treatment was investigated in vitro AA?like hair follicle organ model and in vivo C3H/He J AA mice model.Therapeutic mechanisms were detected by q PCR,Western blot and immunofluorescence.Results:AA?like hair follicle organ in vitro model was successfully established by culturing mouse vibrissa follicles with interferon?γ(IFN?γ).The AA?like symptoms were relieved when IFN?γinduced AA in vitro model was cocultured with HF?MSC for 2 days.In addition,when skin grafted C3H/He J AA mice models were injected with 10~6 HF?MSCs once a week for 3 weeks,the transcription profiling and immunofluorescence analysis depicted that HF?MSC treatment significantly decreased mouse hair loss and reduced inflammation around hair follicle both in vitro and in vivo.Conclusions:This study provides a new therapeutic approach for alopecia areata based on HF?MSC toward its future clinical application.