Study On Urinary Microbiome In Patients With Calcium Oxalate Kidney Calculi

Purpose:The urine microbial composition of patients with calcium oxalate renal calculi was detected by 16 S rRNA amplicon sequencing technique to explore the correlation between calcium oxalate kidney stones and urine microbiome.Methods: A total of 15 patients with occasional kidney stones(OS)and 14 patients with recurrent kidney stones(RS)were included in this study,and 14 healthy people were recruited as the control group(HC).Urine samples from three groups were collected respectively,and the composition of urine microbiome was detected by 16 S rRNA amplicon sequencing technology.Results: The microbial diversity of urine in healthy control group was significantly lower than that in calcium oxalate kidney stone group(P< 0.01).There was also significant difference in urinary microbial structure between calcium oxalate kidney stone patient group and healthy control group(P < 0.01).Bacteria that promote the aggregation of calcium oxalate crystals were found in the group of patients with calcium oxalate kidney stones(KS),such as Bacteroides,Klebsiella and Proteus.In the healthy control group,anti-inflammatory bacteria were found,such as Veillonella and Prevotella.There was no significant difference in the diversity of urine microbiome and the structural distribution of urine microbiome between OS and RS,but the RS group was mainly composed of enterobacteria,Klebsiella and Proteus.Through KEGG function prediction analysis,it was found that there were differences in cell growth and death,excretory system and translation function between calcium oxalate kidney stone patient group and healthy control group.Conclusion: This study found that 16 S rRNA amplicon sequencing technology can be used as a reliable means to detect urine microbiome;There were significant differences in urine microbial diversity and microbial structure distribution between patients with calcium oxalate renal calculi and healthy people;The presence of Bacteroides,Klebsiella and Proteus may be an important factor for the formation of calcium oxalate kidney stones.