Effects Of Different Anticoagulants And Dosages On Preparation Of Platelet-rich Plasma And Treatment Of Knee Osteoarthritis

Purpose: Platelet rich plasma(PRP)has been widely used in the treatment of knee osteoarthritis(KOA)at home and abroad,but its efficacy and preparation methods have been controversial,and improper application of anticoagulants may cause adverse effects such as joint swelling and pain after treatment.The aim of this study was to investigate the effects of different anticoagulant types and doses on the formulation of PRP and its prepared PRP on the therapeutic effects of KOA.Methods: From April 2019 to April 2020,288 patients treated with PRP in our hospital were retrospectively analyzed,including 118 male patients and 170 female patients with a mean age of 57.03 years(38-79years).The anticoagulants used to prepare PRP,the ratio of anticoagulant/blood configuration and the number of patients treated with KOA were: 4% sodium citrate(0.6 ml/10 ml,74 patients;1 ml/10 ml,73patients),and heparin(0.1 ml/10 ml,72 patients;0.2 ml/10 ml,69patients),respectively.The items evaluated in this study were platelet counts in patients’ whole blood and autologous PRP,the occurrence of adverse effects in the short term after treatment,and the Western Ontario and Mc Master Universities Osteoarthritis Index(WOMAC)scores before and after treatment.WOMAC)scores.Results: PLT counts in BR were compared with PLT counts in PRP in each group using paired-samples t-test.4% SC: 0.6 ml group [B-PLT :(207.15±49.83),P-PLT:(932.20±234.98)],)4% SC: 1 ml group [B-PLT :(205.63±49.51),P-PLT:(926.55±213.84)],Heparin: 0.1 ml group[B-PLT :(208.24±39.68),P-PLT:(756.43±220.29)],Heparin: 0.2 ml group [B-PLT :(203.54±38.45),P-PLT:(757.68± 213.49)],the differences were statistically significant(all P < 0.001),and the differences between groups were significant and statistically significant(P < 0.001),the incidence of knee swelling and pain after PRP treatment in the low-dose group and the high-dose group were significant and statistically significant(all P < 0.001),the WOMAC scores of the four groups before and 6 months after treatment were significantly different and statistically significant(All P < 0.001),no significant difference between the 4 groups(P > 0.05),and the WOMAC scores of the 4 groups before and 12 months after treatment were only significantly different between the 4% SC: 0.6 ml group and the 4% SC: 1 ml group,with statistical significance(all P < 0.001),no significant difference between the 4 groups(P > 0.05).Conclusions: PRP can effectively relieve the symptoms of KOA patients.Meanwhile,the low-dose anticoagulant group: 4% sodium citrate to whole blood ratio of 0.6 ml/10 ml was a better choice of anticoagulant and dose for the preparation of PRP,and the PRP prepared by this group of anticoagulants not only had higher PLT concentration,but also achieved better medium-and long-term efficacy in avoiding the occurrence of knee swelling and pain after treatment.