| Objective: To explore the correlation between serum Galectin-3(Gal-3)and C-type lectin like receptor 2(CLEC-2)levels of Ischemic stroke(IS)and the nature of carotid artery plaque from ischemic stroke patients.Methods: Collection of Cheng De medical college affiliated hospital neurology 72 h disease and cooperate to improve the neck vascular ultrasound in acute ischemic stroke(Acuteischemicstroke,121 AIS patients were divided into endometrial thickening group(31 cases),non-vulnerable plaque group(41 cases)and vulnerable plaque group(49 cases)according to the results of cervical vascular ultrasonography.In addition,32 healthy subjects without IS or carotid intima thickening during the same period were selected as the control group.Serum Gal-3 and CLEC-2 levels were determined by enzyme-linked immunosorbent assay(ELISA).Data of gender,age,body mass index(BMI),smoking history,alcohol consumption history,total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),creatinine,Gal-3,and CLEC-2 were collected.To compare whether there was statistical significance in the difference of each index among the groups.Spearman correlation analysis was used to investigate the effects of carotid atherosclerosis,also known as carotida,logistic regression analysis was used to evaluate the independent risk factors of CAS vulnerable plaques,and receiver operating characteristic curve(ROC)was plotted to evaluate the optimal cut-off point of serum Gal-3 and CLEC-2 in predicting the occurrence of CAS vulnerable plaques in patients.Results:1.Comparison of general data and serological indexes among the four groups: there were no significant differences in gender,age,BMI,smoking history,drinking history,TG,TC,HDL-C,LDL-C and creatinine(P>0.05).2.The serum levels of Gal-3 and CLEC-2 in control group,endometrial thickening group,non-vulnerable plaque group and vulnerable plaque group were statistically significant(P<0.001).The expression levels of serum Gal-3 and CLEC-2 in four groups were further pairwise compared,and the differences were statistically significant(P<0.05).3.Serum Gal-3 in control group,endometrial thickening group,non-vulnerable plaque group and vulnerable plaque group were 1.29±0.50ng/m L,1.65±0.52 ng/m L,2.11±0.53ng/m L and 2.56±0.55ng/m L,respectively.The CLEC-2 values were 2.90±0.46 ng/m L,3.22±0.56 ng/m L,3.55±0.44 ng/m L and 4.05±0.49 ng/m L,respectively.It showed an increasing trend,the difference was statistically significant(P<0.001).4.Spearman correlation analysis showed that serum Gal-3(r=0.536,P<0.001)and CLEC-2(r=0.577,P<0.001)levels were positively correlated with CAS plaque vulnerability.5.Logistic regression results showed that Gal-3(OR=8.408,95%CI:3.161-22.366,P<0.001),CLEC-2(OR=15.137,95%CI: 4.543 ～50.433,P<0.001)were independent risk factors for CAS vulnerable plaques.6.ROC curve analysis results showed that the optimal cut-off point of serum Gal-3 level to predict the occurrence of CAS vulnerable plaques was2.010ng/L,the area under ROC curve was 0.831(P<0.05),and the sensitivity and specificity were 85.7% and 66.3%.The optimal cut-off point for predicting CAS vulnerable plaque was 3.431ng/L and the area under ROC curve was 0.857(P<0.05).The sensitivity and specificity were 95.9% and61.5% respectively.Conclusions:Serum Gal-3 and CLEC-2 levels are positively correlated with CAS plaque vulnerability in AIS patients,which are risk factors for CAS vulnerable plaque occurrence in AIS patients,and have good predictive value. |
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