The Expression And Clinical Significance Of Replication Factor C Subunit 2(RFC2) In Gastric Adenocarcinoma

Objective The incidence and mortality of gastric cancer have been high.It is of great significance to find effective diagnostic and therapeutic targets.Replication factor C subunit 2(RFC2),as a member of the RFC protein complex,is an important protein that plays a role in DNA replication and cell cycle regulation by binding DNA and transcription factors.It has been shown to be abnormally expressed in a variety of tumors,but there is no relevant research in gastric cancer.This article intends to explore the expression and clinical significance of RFC2 in gastric adenocarcinoma.Methods The expression of RFC2 in gastric cancer tissues was analyzed by bioinformatics technology.The expression of RFC2 protein in 80 cases of gastric adenocarcinoma,adjacent tissues and normal gastric mucosa tissues was detected by immunohistochemistry and Western blot.The clinicopathological data such as gender,age,TNM stage and number of lymph node metastasis were collected.The prognosis of patients was obtained through various follow-up approaches,and the survival curve of patients was drawn.The relationship between relevant clinical data and RFC2 expression was analyzed by univariate and multivariate analysis,and the prognostic factors were analyzed.The gastric cancer cell lines SGC-7901 and BGC-823 were treated with RFC2 inhibitor xanthohumol,and the effect of RFC2 on the viability of gastric cancer cells was analyzed by CCK8 assay.The effect of RFC2 on the proliferation of gastric cancer cells was analyzed by plate clone formation assay,and its possible mechanism was further explored by consulting relevant literature and WB experiments.Results Bioinformatics analysis showed that RFC2 was highly expressed in gastric cancer.Immunohistochemistry and WB results showed that RFC2 was highly expressed in gastric adenocarcinoma tissues,and was significantly higher than that in adjacent and normal gastric tissues.Among them,the number of lymph node metastasis,differentiation degree,TNM stage and other related pathological features were related to the high expression of RFC2,and the difference was statistically significant(P < 0.05).Cox model analysis showed that TNM stage,differentiation degree and RFC2 high expression were independent risk factors affecting the prognosis of patients with gastric adenocarcinoma,and the differences were statistically significant(P < 0.05).In gastric cancer cell lines SGC-7901 and BGC-823 treated with RFC2 inhibitors,CCK8 and plate colony formation experiments showed that low expression of RFC2 inhibited the viability and proliferation of gastric cancer cells(P < 0.05).WB results showed that the transcription factor forkhead box O1(FOXO1)protein expression was increased in gastric cancer cells with low expression of RFC2(P < 0.05).Conclusion RFC2 is highly expressed in gastric adenocarcinoma tissues,and its expression is related to the number of lymph node metastasis,differentiation degree and TNM stage.RFC2 promotes the occurrence and development of gastric cancer cells by inhibiting the expression of FOXO1 protein.RFC2 may play a key role in the occurrence and development of gastric adenocarcinoma,and has a certain impact on the prognosis of patients,which may become a new tumor marker.