Th17/Treg Related Cytokines Imbalance Is Involved In The Pathogenesis Of Preeclampsia

Objective To explore the role of Th17/Treg cell related cytokines imbalance in the pathogenesis of preeclampsia.Methods The Serum specimens of 50 preeclampsia pregnant women and 30 normal pregnant women were collected,and the concentrations of IL-23 and IL-2R in serum were determined by ELISA.In addition,the concentrations of IL-6 and IL-10 in serum was determined by cytometric bead array(CBA).The placental and decidual tissues of6 normal pregnant women and 10 preeclampsia pregnant women were further verified by QPCR and immunohistochemical methods.Results The serum concentrations of IL-10,IL-2R,IL-23 and IL-6 in preeclampsia women were higher than those of normal women(P < 0.05).The results were as followed:preeclampsi:2.02,700.37,101.75,5.22(pg/m L);control:0,360.58,83.28,2.69(pg/m L).The results of QPCR showed that there was no significant difference in the expression of IL-23 m RNA in placenta and decidua of preeclampsia patients(P > 0.05).The expression level of IL-10 m RNA in placenta and decidua of preeclampsia women was lower than that of normal women and the difference was statistically significant(P< 0.05).The expression level of IL-2R m RNA in placenta and decidua of preeclampsia women was higher than that of normal women(P < 0.05).Immunohistochemical results showed that the expression of IL-10 in placenta and decidua of preeclampsia women was lower than that of normal women,with a statistically significant difference(P <0.05).The expression of IL-2R and IL-23 in placenta of preeclampsia women was higher than that of normal women,with a statistically significant difference(P < 0.05),while the expression of IL-23 and IL-2R in decidua of the two groups had no statistically significant difference(P > 0.05).Conclusion Compared with normal pregnant women,the expression levels of IL-10,IL-23 and IL-2R are different in preeclampsia pregnant women,which may be related to Th17/Treg immune imbalance,suggesting that the related cytokines changes mediated by Th17/Treg immune imbalance may be involved in the pathogenesis of preeclampsia.