| Background: Lung cancer is the most common malignant tumour of the respiratory system originating from the mucosal glands of the bronchi and alveolar epithelium,of which about 85% is non-small cell lung cancer,with the highest prevalence and mortality rate in China.Patients with early stage lung cancer usually do not have typical symptoms such as coughing,haemoptysis and dyspnoea,which leads to insufficient awareness of the disease among the general public,and when detected,most of them are already at an advanced stage,thus greatly affecting the survival time and quality of patients and imposing a heavy financial burden on their families.Chinese herbal medicine has a deep historical heritage in clinical treatment in China,and with the rapid development of modern pharmacology,the study of active substances in Chinese herbal medicine has been deepened to the molecular level.The chemical composition of Chinese herbs is complex,and the active substances in the anti-tumour field contain terpenoids and alkaloids,saponins,flavonoids,polysaccharides,etc.Matrine is an alkaloid extracted from the traditional Chinese herbal medicine family of bitter beans and has been shown to have a variety of pharmacological effects,including antiinflammatory,anti-viral,anti-fibrotic and cardiovascular protection.In particular,it has been reported in the treatment of several tumour types.The aim of this study is to preliminarily investigate the molecular biological mechanisms involved in the migration and apoptosis of lung adenocarcinoma cells A549 in vitro by matrine,and to provide a theoretical basis for the clinical use of matrine in the treatment of patients with nonsmall cell lung cancer.Objective:To investigate the effects of different concentrations of matrine on the apoptosis and migration ability of human lung adenocarcinoma cells A549 and the molecular mechanism.Methods: A549 cells cultured in medium containing 0.2,0.8,1.2 and 2.4 mg/m L of graded matrine were used as the experimental group,and cells cultured in medium without the drug were used as the control group.The expression of apoptosis-related genes Bcl-2,Survivin and Caspase-9 in each group of cells was detected by RT-q PCR,and the expression of apoptosis-related proteins Bcl-2,Survivin and Caspase-9 in each group of cells was detected by Western blotting.Results: 1.The results of the cell scratch assay showed that the 24-h cell migration rates of the matrine(0,0.2,0.8,1.2 and 2.4 mg/m L)groups were(42.95±8.01)%,(36.25±4.86)%,(22.73±6.33)%,(14.73±3.52)%,(6.33±3.18)%.One-way ANOVA showed significant differences(P<0.01)in the migration rates between the experimental and control groups for 0.8,1.2 and 2.4 mg/m L A549 cells,and the migration rates decreased with increasing drug concentration gradients.2.The results of RT-q PCR showed that the expression of Survivin m RNA and Bcl-2 m RNA in A549 cells was down-regulated and that of Caspase-9 m RNA was up-regulated in the 0.8,1.2 and 2.4mg/m L matrine groups(P<0.01).3.The western blotting results showed that 0.8,1.2 and 2.4 mg/m L of matrine could down-regulate the expression of apoptosis inhibitory protein Survivin and Bcl-2 and up-regulate the expression of pro-apoptotic protein Caspase-9 in A549 cells(P<0.01).Conclusion: matrine effectively inhibited the migration ability of lung adenocarcinoma A549 cells in vitro and increased Caspase-9 gene and protein expression,inhibited Bcl-2,Survivin gene and protein expression,and promoted apoptosis of A549 cells. |
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