| Objective: The report collected the pharynx swab specimens from adult respiratory infected patients from a Grade A hospital in Nanjing from November2020 to October 2021,developed virus profile screening and studied clinical features and virus infection characteristics.The essay analyzed the genetic characteristics of rhinovirus with the highest virus infection rate and its correlation with clinical features,and provided some scientific data for understanding the characteristics and prevention of adult respiratory virus infection in Nanjing.Methods: The subjects of this experiment were obtained from 424 throat swab specimens from patients with respiratory infections collected by the a tertiary hospital in Nanjing area from2020 to October 2021.Nucleic acid was extracted from 424 throat swab samples collected,and fluorescence quantitative PCR was used to screen the proposed nucleic acids for 15 common respiratory viruses.For initially screened HRV positive samples,the study amplified and sequenced the HRV-VP4 / VP2 by using the nest PCR method,and analyzed phylogenetic tree and homology of the sequence obtained,at the same time,virus isolation was performed on initial HRV positive samples,and extracted the nucleic acid from samples isolated successfully,the application of fluorescence quantitative PCR and nest PCR were used to identify virus and determine the whole genome sequence of virus.Results: A total of 424 throat swabs were collected from patients with respiratory viral infections and respiratory viruses were detected in 116 throat swab samples.The overall detection rate of virus was 27.36%,and the highest detection rate was HRV(9.43%).Among the 424 cases of respiratory viral infections,including 269 males and 155 females,there had no difference in gender distribution(P>0.05).Patients were divided into four groups by age,with the highest infection rate in the 40-60 years old group,but there was no correlation between viral infection and age.Respiratory viral infections in adults,with higher infections in autumn and winter than in spring and summer.There was no statistically significant difference in the clinical manifestations of respiratory viral infections compared with those without viral infections(P<0.05),but white blood cell count and percentage of basophils may be correlated with viral infection in the clinical test indicators(P<0.05).Partial gene sequences of 33 rhinovirus VP4/VP2 were successfully sequenced,among them,HRV-A contained 11 serotypes,namely HRV,HRV-A1,HRV-A9,HRV-A12,HRVA22,HRV-A24,HRV-A29,HRV-A41,HRV-A49,HRV-A59,HRV-A68,HRV-A78;then HRV-B contained 7 serotypes,respectively HRV-B6,HRV-B27,HRV-B42,HRV-B48,HRV-B92,HRV-B79,HRV-B101,;lastly HRV-C contained 3 serotypes,HRV-C15,HRV-C 1,HRV-C6,and HRV-8,one strain was untyped.Conclusions:1.In this study,13 kinds of(type)respiratory viruses could be detected in pharyngeal swabs of adult respiratory tract infection in Nanjing,and the detection rate reached 27.36%(116 / 424),mainly involving HRV,Flua,RSV,ADV,HCOV-OC 43 and other viruses.The highest detection rate was HRV,reaching9.43%(40 / 424).2.There was no association between different age,gender and respiratory virus infections,and respiratory viruses showed seasonal distribution,higher in autumn and winter than in spring and summer.3.There was no obvious difference in the clinical manifestations between respiratory infection virus-positive patients and respiratory infection virus-negative patients.4.HRV was the main pathogen in adult patients with respiratory viral infections in Nanjing from 2020 to October 2021,and the three genotypes of HRV-A,B,and C were co-prevalent. |
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