Expression Of Long Non-coding RNA SOX2OT In Esophageal Squamous Cell Carcinoma And Its Relationship With Radiosensitivity

Objective: To explore the expression difference of long-chain non-coding RNASOX2 OT in esophageal squamous cell carcinoma(ESCC)tissues and adjacent normal tissues and its relationship with clinicopathological features of patients with ESCC,to detect the expression difference of SOX2 OT in peripheral blood of ESCC patients and its relationship with radiosensitivity,and to study the biological role of SOX2 OT in esophageal squamous cell carcinoma by cell experiment.Methods: Collect the cancer tissues of patients with esophageal squamous cell carcinoma diagnosed pathologically and treated by radical surgery,and detect the difference of tissue expression by q RT-PCR,and analyze the relationship with clinical pathological features of ESCC patients.The peripheral blood of ESCC patients who did not receive any treatment and underwent radical radiotherapy before and one month after radiotherapy,and the peripheral blood of normal people were collected.The expression difference of SOX2 OT in peripheral blood was detected by q RT-PCR.By comparing the results of barium meal examination and CT examination of esophageal cancer patients before and after radiotherapy,according to the evaluation criteria of solid tumor curative effect: complete remission(CR),partial remission(PR),stable disease(SD),progressive disease(PD)Patients were divided into two groups,the radiotherapy sensitive group including CR and PR,and the radiotherapy resistant group including SD and PD.The correlation between the expression of SOX2 OT and its shortterm curative effect was analyzed.CCK-8,cell scratch and clone formation experiments were used to study the effect of SOX2 OT expression on proliferation,migration and radiotherapy sensitivity of esophageal squamous cell carcinoma.Results: First.Compared with the normal tissues adjacent to cancer,SOX2 OT is highly expressed in ESCC tissues,and its expression level is related to T stage of tumor,but not related to age,sex,tumor length,lymph node metastasis and tissue differentiation degree of patients.Second.The expression of SOX2 OT in peripheral blood of patients with esophageal squamous cell carcinoma is higher than that of healthy people.For ESCC patients undergoing radical radiotherapy,the expression level of SOX2 OT in radiotherapy sensitive group is lower than that before radiotherapy,but there is no significant difference in radiotherapy resistant group before and after radiotherapy.Comparing the expression of SOX2 OT in peripheral blood of radiotherapy sensitive group and radiotherapy resistant group before radiotherapy,the results show that the expression level of sox2 ot in radiotherapy resistant group is higher.Third.CCK-8 experiment proved that inhibition of SOX2 OT could reduce the clone formation rate of ESCC cells,and the cell proliferation ability was lower after radiotherapy.Cell scratch experiment showed that down-regulation of SOX2 OT could inhibit the migration and repair ability of cells.After clone formation experiment,down-regulation of SOX2 OT expression level found that the clone rate of cells decreased.After different radiation treatments,the cell viability of both groups decreased,especially in si-SOX2 OT group.Conclusion: First.Lnc RNASOX2 OT is highly expressed in ESCC,and the expression level is related to tumor T stage.Second.The expression of SOX2 OT in peripheral blood of ESCC patients is high,and the expression level is related to the recent radiotherapy effect of the patients.Third.Downregulating the expression level of SOX2 OT can inhibit the proliferation,migration and repair of ESCC cells,and increase the radiosensitivity of ESCC cells.