Relationship Between The Expression Of ARHGAP25 And RhoA In Non-small Cell Lung Cancer And Vasculogenic Mimicry

Purposes:Vasculogenic mimicry(VM)is a recently identified pattern of blood supply to tumor tissues.It has long been considered a functional element in the metastasis and prognosis of malignant tumors.Both Rho GTPase-activating protein 25(ARHGAP25)and Ras homolog family member A(Rho A)are effective predictors of tumor metastasis.In this study,we examined the expression levels of ARHGAP25 and Rho A and the structure of VM in non-small cell lung cancer(NSCLC).At the same time,we used cytologyrelated experiments to explore the effect of ARHGAP25 on the migration ability of tumor cells.The relationship of each of ARHGAP25,Rho A and VM with the clinicopathological parameters of patients and the survival time of NSCLC patients after surgery was analysed.Methods:A total of 130 well-preserved NSCLC and associated paracancerous tumor-free tissues were obtained.Cell colony formation,wound healing,and cytoskeleton staining assays were used to analyze the effect of ARHGAP25 on the proliferation and migration ability of NSCLC cells.Immunohistochemical staining was used to determine the positivity rates of ARHGAP25,Rho A,and VM.Statistical software was used to examine the relationships between the three factors and clinical case characteristics,overall survival,and disease-free survival.Results:ARHGAP25 positivity rates in NSCLC and paracancerous tumor-free tissues were 48.5%and 63.1%,respectively,whereas Rho A positivity rates were 62.3% and 18.5%,respectively.VM structures were not present in the paracancerous tumor-free tissues,and the positive rates of VM in NSCLC were 36.9%.ARHGAP25 had a negative relationship with Rho A and VM,whereas Rho A and VM had a positive relationship(P< 0.05).ARHGAP25,Rho A,and VM affected the prognosis of patients with NSCLC(P < 0.05)according to Kaplan–Meier of survival time and Cox regression analyses.In addition,cell colony formation,wound healing,and cytoskeleton staining assays confirmed that decreasing ARHGAP25 expression increased proliferation and migration of NSCLC cells.In addition,cell colony formation,wound healing,and cytoskeleton staining assays confirmed that decreasing the expression level of ARHGAP25 increased the proliferation and migration ability of NSCLC cells.Conclusions:(1)ARHGAP25 low expression,Rho A overexpression and the presence of VM promote the progression of NSCLC.(2)ARHGAP25 low expression and Rho A overexpression promote the formation of VM structures.(3)ARHGAP25 low expression,Rho A overexpression and the presence of VM can lead to poor prognosis.