Mechanism Of IMP2 Regulating Alternative Splicing Of Esophageal Cancer-related Genes

Objective:Studying how IMP 2 affects the initiation and progression of esophageal cancer and achieves this by regulating gene expression and alternative splicing may help provide an effective rationale for the diagnosis and treatment of esophageal cancer.Methods:RT-q PCR,RNA-Seq were used to study the overexpression and transcriptome sequencing of IMP 2 in human esophageal cancer cell line ECA109,analyze the data of the whole genome,and explore the differentially expressed genes and alternative splicing genes regulated by IMP 2.Results:1.After IMP 2 overexpression,158 genes caused significant differential expression,among which 81 genes were up-regulated and 77 genes were downregulated(FC ≥ 2 or ≤ 0.5,P<0.01).IMP 2 was found by GO and KEGG functional enrichment,which affected the occurrence and development of esophageal cancer by regulating the differentially expressed genes,and may not be significant.2.Genes with variable splicing levels that changed significantly after IMP 2overexpression were enriched for positive cell regulation,apoptosis,regulation of protein stability,posttranslational protein modification,gene expression,and the main enriched signaling pathways were cell proliferation and apoptosis.In the cell proliferation pathway,21 alternative spliced genes were found after IMP 2 overexpression,including ADAMTSL4,TRIO,PLEKHG2,RBCK 1,ABL 1,FAM162 A,ITSN1,ARHGAP4,TNFRSF12 A,MELK,etc.In the apoptosis pathway,23 alternative splicing genes were identified after IMP 2 overexpression,including CDK11 B,OSMR,MRE11 A,MELK,FTSJ2,ARHGEF1,IFI 16,CUL 5,CITED1 and others.Conclusions:IMP2 may play a role in esophageal cancer by regulating the variable splicing of cell proliferation and apoptosis-related genes.