Factors Influencing Oral Health Service Utilization In 150 Children With Acute Lymphoblastic Leukemia And Differential Analysis Of Oral Bacteria Before And After Chemotherapy In 8 Cases

Objective: The aim of the study was to investigate the factors influencing the utilization of oral health services in 150 children with acute lymphocyte leukemia(ALL)and to understand the variability of oral bacteria before and after induction chemotherapy in 8 children with initially diagnosed ALL,and to further analyze the impact of changes in oral flora on oral complications and systemic infection rates.Methods:One hundred and fifty ALL patients were recruited for oral examination and their family members were surveyed by questionnaires,including age,time of diagnosis,whether they used fluoride toothpaste and mouthwash and parents’ knowledge of oral cavity,etc.Eight patients with initial diagnosis of ALL were screened by oral examination as the experimental group,saliva samples were collected before and after induction chemotherapy,and eight healthy children were matched as the control group,and high-throughput sequencing technology was used to The samples were sequenced with 16 sRNA by high-throughput sequencing technology.Results:Logistic regression analysis showed that parents’ oral hygiene awareness,oral attitude,mouthwash use during chemotherapy,and postprandial oral cleaning practices were factors influencing the oral visit rate.The bacterial diversity and richness were statistically different before induction chemotherapy compared with healthy children,with a significant increase in the richness of Phylum Thicket,Phylum Actinomyces,Streptococcus haemolyticus,Prevotella,and Actinomyces spp.and a significant decrease in Neisseria spp.Compared with before and after induction chemotherapy,the species richness of the phylum Actinomycetes increased slightly after induction chemotherapy,but the difference was not significant,and the abundance of the genera Weyrong’s,Prevotella,and Actinomycetes increased.Conclusions:Low utilization of oral health services in ALL children,parents’ knowledge of oral hygiene and oral attitudes are the main influencing factors,and dysbiosis of oral flora before and after chemotherapy with variability,which may become a risk of oral complications or systemic infections,the results of this study may help to develop better personalized infection prevention strategies that will benefit the treatment of ALL and improve the quality of life of the children.