Effect Of Silencing Long Noncoding RNA HOTAIR On Radiosensitivity Of Tongue Squamous Cell Cells

Objective:To investigate the effect of down-regulated expression of long-chain noncoding RNA HOTAIR on the radiosensitivity of tongue squamous cell lines CAL-27 and SCC-9.Methods:Three HOTAIR interference sequences were designed and transfected into tongue squamous cell cells CAL-27 and SCC-9 by lentivirus vector.Realtime quantitative PCR(q RT-PCR)was used to test the efficacy of the intervention..The two kinds of cells were divided into blank group(CAL-27,SCC-9),experimental group(si-HOTAIR CAL-27,si-HOTAIR SCC-9)and negative control group(si-NC CAL-27,siNC SCC-9),and then the above cells were irradiated with 8Gy electron line.Cell proliferation was detected by MTT assay,cell migration was detected by scratch assay,and cell apoptosis was determined by flow cytometry.Results:Between the three lentiviral vectors,si-2 and si-3 down-regulated HOTAIR expression in tongue squamous cell cells CAL-27 and SCC-9.Statistical analysis showed that the interference efficiency of si-3sequence was the highest,which could down-regulate HOTAIR expression by 68.6% in CAL-27 cells and 50.8% in SCC-9 cells(all P < 0.05).After down-regulated lnc RNAHOTAIR in tongue squamous cell cells CAL-27 and SCC-9 cells combined with 8Gy electron line irradiation,compared with control group(si-NC CAL-27,si-NC SCC-9)and blank group(CAL-27,SCC-9),The proliferative ability of the experimental group(siHOTAIR CAL-27,si-HOTAIR SC-9)was significantly decreased(P < 0.05).Cell apoptosis rate increased [(23.87±1.97)%vs.(46.03±2.49)%,(15.99±0.76)%vs.(39.38±2.95)%)] and mobility decreased [65.85% vs.58.82%,47.81% vs.37.78%].There was a significant difference between the two groups(all P < 0.05).Conclusion:Decreased HOTAIR expression can increase the radiosensitivity of CAL-27 and SCC-9 in tongue squamous cell cells.