Diagnostic Value Of LncRNA PVT1 In Liver Fibrosis For Patients With Chronic Hepatitis B

Objective:To analyze the clinical value of serum LncRNA plasmacytoma variant translocation 1(LncRNA PVT1)in the diagnosis of liver fibrosis for patients with chronic hepatitis B,and to provide fresh ideas for exploring the early diagnosis and treatment of liver fibrosis.Methods:A total of 83 study subjects,including 70 HBV-infected patients and 13 healthy individuals,were included in this study.All study subjects had 5 ml of whole blood specimens,and the patients with HBV infection had biochemistry,coagulation function,blood routine,hepatitis B five items,HBV-DNA quantification,tumor index,hepatobiliary,pancreatic and spleen ultrasound or liver CT examination,etc.All patients with HBV infection had liver tissue biopsy.The retained liver tissue specimens were stained with hematoxylin-eosin stain and fibrous Masson stain for Scheuer fibrosis score.All HBV-infected patients were divided into four groups according to liver fibrosis grading:Group A S0(7 cases),group B S1(33 cases),group C S2(22 cases),and group D S3+S4(8 cases).The HBV-DNA load(lg copy number/mL)was divided into three groups according to HBV-DNA load:low viral load(<3),medium viral load(3-5),and high viral load(>5).The expression levels of LncRNA PVT1 in the serum of all study subjects were detected using real-time fluorescence quantitative PCR technique.Statistical analysis:statistical analysis was performed on the data.The measured data were expressed as mean ± standard deviation,and the differences between groups were compared by U test or Kruskal Wallis rank sum test.Pearson or Spearman were used to analyze the correlation of the data.The diagnostic value of serum LncRNA PVT1 was evaluated using ROC curves.The difference was considered statistically significant at P<0.05.Results:Serum expression of LncRNA PVT1 levels elevated with increasing degree of liver fibrosis in patients with chronic hepatitis B.There was a significant correlation between them(P<0.05).Group A,B,C,D were(2.38±0.53)U/L,(2.93±0.57)U/L,(2.95±0.56)U/L,(4.76±2.86)U/L,respectively.There was no significant correlation between LncRNA PVT1 expression and hepatitis B viral load(P>0.05),(3.39±1.71)U/L for low viral load,(2.90±0.71)U/L for medium viral load and(2.90±0.71)U/L for high viral load.After ROC curve analysis,the area under the curve AUC values were S1(0.791),S2(0.622),S3+S4(0.804)when setting the LncRNA PVT1 cut-off values(Cut-off values)to≥2.405,≥2.730 and≥3.800,respectively.Conclusion:1.the expression of LncRNA PVT1 in serum was higher in patients with chronic hepatitis B than in healthy subjects.2.The expression of serum LncRNA PVT1 elevated with the increase of liver fibrosis degree in CHB patients,and there was a positive correlation.3.No significant correlation was found between serum LncRNA PVT1 and HBV viral load in CHB patients.4.The expression level of serum LncRNA PVT1 possessed certain value for the diagnosis of liver fibrosis.