| This study aims to investigate the effect and mechanism of Cur-MMT,a complex of curcumin and montmorillonite,on reducing lipid deposition induced by high lipid in mice,and to provide a reference for animal health and montmorillonite-loaded plant extracts.Experiment 1: The effect of modified montmorillonite loaded with curcumin on body lipid content and growth parameters in high lipid-induced miceIn this experiment,40 6-week-old male C57BL/6N mice were randomly divided into 4 groups: low-lipid group(CT),high-lipid group(HF),high-lipid group with added curcumin(HC),and high-lipid group with added curcumin-loaded montmorillonite(HMC).There were 10 replicates in each group,and each replicate had one mouse.The mice were fed with low-lipid diet,high-lipid diet,or high-lipid diet with curcumin-loaded montmorillonite.The light and dark cycle was 12 hours each.After 1 week of adaptation,the formal experiment was carried out,and the experimental period was 10 weeks.The results showed that compared with the CT group,the final weight of the HF and HC groups increased by 7% and 26.2%,and the average daily weight gain increased by 50% and 60%(P < 0.05),while the final weight of the HMC group decreased by 14.6%,and the average daily weight gain decreased by 46.7%(P < 0.05).In terms of body lipid content,compared with the CT group,the subcutaneous lipid and abdominal lipid in the HF group increased by 94.3%(P < 0.05)and 284%(P <0.05),respectively,while the subcutaneous lipid and abdominal lipid in the HMC group decreased by 37.4%(P < 0.05)and 50.9%(P < 0.05),respectively.The analysis of organ index showed that compared with the CT group,the liver index and kidney index increased by 17.5% and 12.7%,respectively,and both had significant differences(P < 0.05).The HMC group compared with the HF group showed a significant increase in the tibialis anterior muscle index by 35.9% and gastrocnemius muscle index by 19.9%,while decreasing the liver index by 16.3%(P < 0.05).There was no significant difference in subcutaneous lipid and abdominal lipid between the HC and HF groups.Experiment 2: The mechanism of curcumin-loaded montmorillonite in reducing lipid deposition in high lipid-induced mice.In this experiment,30 6-week-old male C57BL/6N mice were randomly divided into 3 groups: low-lipid group(CT),high-lipid group(HF),and high-lipid group with added curcumin-loaded montmorillonite(HMC).There were 10 replicates in each group,and each replicate had one mouse.The mice were fed with low-lipid diet,highlipid diet,or curcumin-loaded montmorillonite-added high-lipid diet.The management of mice feeding was the same as in Experiment 1.After 10 weeks of the experiment,the biochemical indicators,inflammatory factors,and gut microbiota of the experimental mice were measured.The experiment investigated the effects of curcumin-loaded montmorillonite(HMC)on high-lipid diet-induced obesity in mice.The results showed that HMC significantly reduced the levels of total cholesterol,triglycerides,LDL,ALT,insulin,and leptin in serum,and reduced lipid droplet accumulation in liver and adipose tissue.HMC also increased the expression of GLU and Glut4,decreased inflammatory cytokines TNF-α,IL-6,and IL-1β,and promoted the growth of Bifidobacterium while decreasing the abundance of Prevotella in gut microbiota.HMC was found to regulate gut microbiota metabolism pathways and improve the stability of gut microbiota,thereby alleviating high-lipid diet-induced obesity in mice.1.The experiment compared the effects of curcumin-loaded montmorillonite(HMC)and high-lipid diet(HF)on mice with a control group(CT).Blood biochemistry tests showed that HF significantly increased the levels of total cholesterol,triglycerides,LDL,ALT,insulin,and leptin in serum compared to the CT group(P < 0.01),but HMC treatment significantly reduced the levels of TG,LDL,and insulin in serum(P < 0.05)and increased the expression of GLU4(P < 0.01)compared to the HF group.2.The histological examination of liver and adipose tissue showed that HF induced a significant accumulation of lipid droplets in liver cells,while HMC treatment reduced the accumulation of lipid droplets and restored the morphology of liver cells to a more normal state.The diameter of adipose cells was also smaller in the HMC group than in the HF group.3.The experiment also investigated the expression of genes related to thermogenesis and inflammation in adipose tissue.HMC treatment significantly reduced the expression of inflammatory cytokines TNF-α,IL-6,and IL-1β,while increasing the expression of Glut4(P < 0.05)compared to the HF group.However,HMC had no significant effect on the expression of genes related to thermogenesis,such as P-65,PKCθ,and IRS1.4.the experiment used high-throughput sequencing of 16 S rRNA to investigate the effects of HMC on gut microbiota.The results showed that HF significantly increased the abundance of Actinobacteria compared to the CT group,while HMC treatment significantly increased the abundance of Bacteroidetes and decreased the abundance of Firmicutes and Actinobacteria(P<0.01)compared to the HF group.At the genus level,HMC treatment significantly increased the abundance of Bifidobacterium and decreased the abundance of Prevotella compared to the HF group.5.the experiment investigated the effects of HMC on gut microbiota metabolism pathways.The results showed that HMC treatment significantly enriched gut microbiota in the metabolism of methylglyoxal and L-arginine pathways(P < 0.001)compared to the HF group.In conclusion,the experiment demonstrated that curcumin-loaded montmorillonite can effectively alleviate high-lipid diet-induced obesity in mice by reducing serum lipid levels,inhibiting lipid droplet accumulation in liver and adipose tissue,promoting the expression of some thermogenesis-related genes,inhibiting stress-related cytokines,increasing the abundance of Bifidobacterium,decreasing the abundance of Prevotella,and regulating gut microbiota metabolism pathways.These findings provide a theoretical basis for new methods. |
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