| Objective: Ulcerative colitis(UC)is a chronic inflammatory bowel disease disease(IBD)that can cause serious harm to human health,oridonin(Ori)is a diterpenoid isolated from the medicinal plant“Oridonin” in our country,which has obvious anti-tumor and anti-inflammatory activity,it has good effect on many kinds of tumor and inflammatory diseases.The aim of this study was to investigate the protective effect of Ori on dextran sodium sulfate(DSS)-induced UC in mice and its mechanism.Methods: Mice were induced to make a animal model of UC by feeding them with 3%DSS solution,followed by therapeutic intervention of UC mice using Ori,objective to investigate the protective effect of Ori on UC by observing the changes of colonic length,pathological changes and expression of related inflammatory factors and channel proteins.15 male BALB/C mice aged 6-8 weeks were randomly divided into three groups: Control group,DSS group and Ori group with 5 mice in each group.Mice in the Control group were fed with clean water,and mice in the DSS and Ori groups were fed with 3% DSS solution prepared with sterile water for 7 days,ori(2mg/kg)was administered intraperitoneally to mice in the Ori group daily from day 7 for 7 days,at the same time,mice in the Control and DSS groups were given equal amounts of saline intraperitoneally.The body weight,disease activity index(DAI)scores of the three groups were observed.In order to further observe the colon condition of mice,mice(n=15)were euthanized by intraperitoneal injection of pentobarbital sodium(40 mg/kg),and the mice were killed by cervical spine dislocation after successful anesthesia,colon tissue was excised and collected for subsequent experiments.The changes of colon length were observed,and the colon tissues were stained with hematoxylin and eosin(HE)to observe the pathological damage and repair,the levels of inflammatory factors such as Myeloperoxidase(MPO),Malondialdehyde(MDA),Superoxide dismutase(SOD)and Glutathione peroxidase(GSH-PX)in colon were measured by Elisa,Western Blot was used to detect the expression of SIRT1/NF-κ b pathway-related proteins at protein level.The data collected above were analyzed with SPSS 21.0 software.The measurement data were presented as mean ± SD.The differences between groups were compared by unpaired t-test and one-way ANOVA with Tukey test,there was statistical difference in P < 0.05.Results: The results showed that,compared to the Control group,in both DSS group and Ori group,the weight of mice decreased,DAI score increased,the colon shortened,the levels of MPO and MDA increased,the levels of SOD and GSH-Px decreased,the expression of SIRT1 was inhibited and the expression of NF-κB increased(p < 0.05),compared with the DSS group,in Ori group,there were less weight loss,lower DAI score,shorter colon,lower levels of MPO and MDA,lower levels of SOD and GSH-Px,lower levels of SIRT1 and NF-κ B(p < 0.05,p < 0.01),in addition,the pathological inflammation is relatively mild.Conclusion: Ori was able to ameliorate clinical symptoms and pathological lesions in DSS-induced UC mice to some extent,and Ori was able to reduce inflammatory response and oxidative stress in colon tissue of DSS-induced UC mice to some extent,ori may exert its anti-UC activity through SIRT1/NF-κb signaling pathway.These results suggest that Ori may be a new therapeutic target for UC. |
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