Research And Development Of Berberine Hydrochloride Antibacterial Composite Hydrogel

After years of scientific research,it has been found that natural medicinal plants,compared with chemical antibiotics,have significant effect,less toxic side effects,difficult drug resistance,and diverse antibacterial targets.In this paper,a near infrared light（NIR）absorber iron tannic acid and medicinal plant antibacterial ingredient berberine hydrochloride were used to construct an antibacterial compound hydrogel（ATB composite hydrogel）.The ATB composite hydrogel production method is simple to operate,with mild process conditions,and can be prepared with conventional equipment.The specific study contents and results are as follows:（1）In this study,using a natural polymer material agarose as the matrix component of ATB compound hydrogel,and combining tannic acid,ferric chloride and berberine hydrochloride,we constructed a compound hydrogel dressing with berberine hydrochloride as antibacterial agent for the antibacterial properties of wound infection site.The ATB composite hydrogel prepared after screening the ratio of tannic acid to Fe³⁺has good elasticity and rich procability.Scanning electron microscopy and Fourier infrared spectroscopy experiments showed that ATB composite hydrogel has been successfully constructed,and loading berberine hydrochloride does not adversely affect the hydrogel itself.The swelling performance,drug release,photothermal performance and hemolysis of ATB composite hydrogel were studied successively,and the experimental results showed that ATB composite hydrogel has excellent swelling performance,and the highest swelling rate reaching 2510 times in PBS under different p H conditions.Secondly,adding near-infrared light can be similar to the“on/off”to achieve the controllable release of berberis hydrochloride in the ATB composite hydrogel.For 10 min under the near-infrared light,the light absorber in the ATB composite hydrogel component can further convert the light energy into heat energy,and rapidly increase the temperature to more than53℃,so as to achieve the physical damage（thermal damage）to the bacteria.More importantly,the hemolysis rate of the ATB composite hydrogel meets the national safety standards for biomaterials.Therefore,this ATB composite hydrogel can be further used as a wound dressing material.（2）The in vitro antibacterial properties of ATB composite hydrogel were studied by plate coating method and morphological characterization method,and the experimental results showed that the number of colonies treated with ATB composite hydrogel group was reduced by76.85%compared with the control group,and the number of ATB+NIR composite hydrogel group was reduced by 97.15%,showing a significant amount of in vitro antibacterial effect,and the near infrared light irradiation significantly improved the antibacterial effect of ATB composite hydrogel.SEM analysis of ATB compound hydrogel,the analysis results show that the S.aureus treated with ATB compound hydrogel has obvious deformation,can clearly observe many wrinkled cracked bacteria,add ATB+NIR composite hydrogel group showed the best antibacterial effect compared with other groups,S.aureus body basic disintegration and fragmentation,the least complete.From the results of the in vitro antibacterial experiments,it can be seen that the ATB composite hydrogel prepared in this experiment has a better photothermal synergy to enhance the antibacterial effect.（3）The antibacterial experiment of animal wound infection on ATB composite hydrogel showed that ATB composite hydrogel can effectively reduce S.aureus infection at the wound and promote the healing of skin wounds.Because near-infrared light irradiation can realize the controlled release of ATB composite hydrogel to berberine hydrochloride,it can be adjusted according to specific requirements in practical application,which can effectively reduce the local accumulation of effective drugs in the wound caused by continuous release.Second,the histological analysis showed that the ATB composite hydrogel did not cause any damage or lesions to the main organs of the mice.It can be seen that this ATB composite hydrogel has a significant effect in the treatment of infections caused by S.aureus and promoting the healing of skin wounds.It can be applied to functional dressings,drug-loaded hydrogel and other fields,and is expected to provide new ideas and methods for the research and development of intelligent photothermal antibacterial dressings.