Analysis Of Cinnamon Oil Components And Its Mechanism Of Regulating Insulin Resistance On IR-HepG2

With the improvement of people’s living standard and the change of life style,the incidence of diabetes is increasing year by year,which has brought serious impact on human health.However,common hypoglycemic agents have different degrees of side effects,so it has become a research hotspot to find more natural products with hypoglycemic effect.In this paper,cinnamon oil was taken as the research object to explore its specific composition,determine its effect on glucose metabolism in insulin-resistant bodies and its corresponding mechanism,detect its effect on antioxidant capacity in insulin-resistant bodies,and conduct a preliminary study on its effect on AMPK pathway.To explore the potential of cinnamon oil as a natural hypoglycemic drug.The main research results are as follows:（1）Cinnamon oil from Chinese and Vietnam was extracted by steam distillation,respectively.The two kinds of cinnamon oils were bright and clear in color and fragrant,but Chinese cinnamon oil was darker in color and Vietnam cinnamon oil had stronger aroma.Among the two kinds of cinnamon oils,47 compounds were identified within 19.78 min from Chinese cinnamon oil,and the main compounds were trans-cinnamaldehyde 34.834%,（+）-Cyclosativene 20.764%,Epizonarene 10.556%,while 49 compounds were identified from Vietnam cinnamon oil.The main compounds were trans-cinnamaldehyde 58.750%andα-pinene 10.146%.（2）HepG2 cells were used as experimental materials,and the cell survival rate and glucose intake were taken as indicators.The optimal modeling conditions of IR-HepG2 cells were obtained as 10^-5mol/L bovine insulin medium for 24 h,and insulin resistance was stable within 60 h after modeling.（3）By measuring the glucose intake and glycogen content of IR-HepG2 cells after treatment with different concentrations of cinnamon oil,it was found that cinnamon oil could increase the glucose intake and glycogen content of IR-HepG2cells to varying degrees,which suggested that cinnamon oil could improve insulin resistance by enhancing the glycogen synthesis ability of glucose.（4）The activities of superoxide dismutase（SOD）and glutathione peroxidase（GSH-PX）and the content of lipid peroxidation product malonydialdehyde（MDA）in IR-HepG2 cells treated with different concentrations of cinnamon oil were detected.The results showed that cinnamon oil could increase the activities of SOD and GSH-Px and decrease the content of MDA in different degrees.It can improve the total antioxidant stress ability of IR-HepG2 cells and alleviate insulin resistance.（5）Western blotting was used to determine the expression levels of some AMPK related pathway proteins（P-AMPKα（Thr172）,P-AKT1（Ser473）,P-GSK-3β（Ser9）,P-FoxO1（Ser256）and PEPCK）in IR-HepG2 cells after cinnamon oil treatment.It was found that cinnamon oil significantly upregulated the protein expression levels of P-AMPKα（Thr172）,P-AKT1（Ser473）,P-GSK-3β（Ser9）and P-Foxo1（Ser256）in IR-HepG2 cells,and down-regulated the protein expression levels of PEPCK,thereby promoting liver glycogen synthesis and inhibiting liver gluconeogenesis.It can alleviate the insulin resistance of IR-HepG2 cells.According to the above experiments,cinnamon oil can alleviate insulin resistance by regulating glucose intake,glycogen content,antioxidant activity and AMPK pathway related protein expression of IR-HepG2 cells,which can be used as a natural hypoglycemic substance for the subsequent development and promotion of hypoglycemic products.