Expression And Mechanism Of MiR-429 And NRG1 In Pancreatic Cancer

Objective:This study intends to detect the expression of micro RNA-429(mi R-429)and neuregulin 1(NRG1)in pancreatic cancer tissues and adjacent tissues,and to study the correlation between them,so as to determine the correlation between mi R-429 and NRG1,and to explore relevant theoretical basis for the diagnosis and targeted therapy of pancreatic cancer.Methods:1.Using public databases(such as Gene Expression Omnibus(GEO),Cancer Genome Atlas(TCGA),star Base:ce RNA and other databases)to analyze the expression of mi R-429 and NRG1 in pancreatic cancer tissues and adjacent tissues.2.The expression levels of mi R-429 and NRG-1 were detected by real-time fluorescence quantitative polymerase chain reaction(RT-PCR)in pancreatic cancer tissues and adjacent tissues.3.The expression of NRG1 in pancreatic cancer tissues and adjacent tissues was detected by Western Blot.Results:1.Through GEO,TCGA and star Base,we found that:(1)The expression of mi R-429 was significantly up-regulated in pancreatic cancer tissues compared with that in adjacent tissues.(2)The expression of NRG-1 in pancreatic carcinoma was lower than that in adjacent tissues.And the decrease of NRG1 expression was significantly correlated with the shortening of overall survival.(3)The expression of mi R-429 was negatively correlated with that of NRG-1 in pancreatic cancer.Through bioinformatics analysis,it was found that:mi R-429 can bind to NRG1 gene and promote the proliferation,metastasis and invasion of pancreatic cancer cells by targeting NRG1 gene.In summary,it can be inferred that mi R-429 is an oncogene in pancreatic cancer,while NRG1 is a tumor suppressor gene in pancreatic cancer.2.RT-PCR detection results:The expression of mi R-429 in pancreatic cancer tissues was higher than that in adjacent tissues(P<0.05),and the expression of NRG1 gene in pancreatic cancer tissues was lower than that in adjacent tissues(P<0.05).3.Results of Western Blot:The expression level of NRG1 in pancreatic cancer tissues was significantly lower than that in adjacent non-cancerous tissues(P<0.05).Conclusion:The expression of mi R-429 is significantly up-regulated and the expression of NRG-1 is significantly down-regulated in pancreatic cancer tissues.mi R-429 was positively correlated with NRG1 in pancreatic cancer.Through biological information analysis,mi R-429 can combine with NRG1 gene,and it may promote the growth,migration and invasion of pancreatic cancer cells by targeting NRG1 gene;mi R-429 is an oncogene in pancreatic cancer,and the NRG1 gene is a tumor suppressor gene in pancreatic cancer.