| Objective:The effects of transcranial direct current stimulation(tDCS)on motor function,dendritic spine growth and Glu R2(glutamatergic receptor 2)in rats with cerebral infarction were investigated,and the mechanism of Glu R2 in the application of tDCS for cerebral infarction was further elucidated to provide a solid theoretical basis for the use of tDCS in clinical treatment.Method: Thirty-two SPF adult SD male rats,weighing approximately 250-280 g,with middle cerebral artery embolism(MCAO)and a neurobehavioural score of 2-3 were randomly divided into sham(sham),MCAO,anodal transcranial direct current stimulation(a-tDCS)and cathodal transcranial direct current stimulation(c-tDCS)groups,8 rats in each group.The sham group of rats were only cut through the skin to separate the tissue without inserting the thread plugs.The MCAO model was established in the MCAO,a-tDCS and c-tDCS groups.The sham and MCAO groups were sham stimulated with electrodes,a-tDCS with anodal stimulation,and ctDCS with cathodal stimulation.(1)The neurobehavioural scores of each group of rats were assessed before modelling,3 days after modelling and 12 days after treatment using the Longa neurobehavioural scale;(2)The changes of infarct foci and their surrounding dendritic spines in brain tissue sections of each group of rats were observed 3 days after modelling and 12 days after treatment using silver-loving staining;(3)The expression of different subtypes of α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid(AMPA)receptors in each group of rats were detected 3 days after modelling and 12 days after treatment using Western-blot.The expression of different subtypes of α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid(AMPA)receptors was examined by Western blot 3 days after modeling and 12 days after treatment.(4)Statistical analysis of the data was performed using SPSS23.0.Results:(1)Neurobehavioural scores of rats in each group: 3 days after modelling,there was a statistical difference between the MCAO,a-tDCS and c-tDCS groups compared to the sham group(P < 0.01),but no statistical difference between the three groups(P > 0.05).12 days after treatment,there was a statistical difference between the a-tDCS and c-tDCS groups compared to the MCAO group(P < 0.001),and between the a-tDCS and c-tDCS groups(P < 0.05).The neurobehavioural scores of the rats in the sham group were 0 in the three time periods before modelling,3 days after modelling and 12 days after treatment.(2)Argenophilic staining of dendritic spines in the peri-infarct tissue: 3 days after modelling,the number of infarct foci and peri-infarct dendritic spines in the MCAO,a-tDCS and c-tDCS groups were not statistically different(P > 0.05).After 12 days of treatment,the number of dendritic spines in the periinfarct tissue of the a-tDCS and c-tDCS groups was statistically different from that of the MCAO group,and the number of dendritic spines in the a-tDCS and c-tDCS groups was statistically different from that of the MCAO group(P < 0.05).(3)Western-blot assay to detect the expression of different isoforms of AMPA receptor protein: after 12 days of treatment,the expression of Glu R2 increased in the a-tDCS and c-tDCS groups compared with the MCAO group,and the difference was statistically significant(P < 0.01),and the expression of Glu R1 decreased after treatment compared with that before treatment,and the difference was statistically significant(P < 0.01),Glu R3 and Glu R4 were not statistically different(P > 0.05).Conclusions:(1)tDCS can reduce neurobehavioural scores and promote motor function recovery in rats with cerebral infarction;(2)tDCS can promote the growth of dendritic spines,enhance neuronal connections and increase synaptic plasticity in brain tissue of rats with cerebral infarction;(3)tDCS can affect the changes of Glu R2 isoforms in brain tissue of rats with cerebral infarction and its surrounding brain tissue.promote dendritic spine growth and improve neurological functional status. |
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