Effect Of SishenPill On Serum VIP,MOT,SP And 5-HT Levels In Rats With Diarrhea-Predominant Irritable Bowel Syndrome With Spleen-Kidney Yang Deficiency Syndrome

Irritable Bowel Syndrome（IBS）is a disease with the main symptoms of abdominal pain,abdominal distension and changes in defecation status.Its etiology is complex,the course of the disease is slow,and it is not easy to be cured,so it affects the quality of life of patients for a long time.It is divided into four types,diarrhea type,constipation type,mixed type,and indeterminate type.According to statistics,IBS-D is more common in China,and IBS-D is mainly characterized by increased stool frequency but basically normal stool volume,and its incidence accounts for 40.83%in irritable bowel syndrome^[1].Modern medical research on its pathogenesis mainly includes psycho-psychological factors,diet-related factors,visceral hypersensitivity,infectious immune factors,gastrointestinal motility disorders,gut-related flora factors,intestinal permeability abnormalities,etc.,but its pathogenesis has not been clarified,so there has been no specific drug in western medicine treatment,and treatment is limited to clinical symptomatic medication,such as 5-HT₃receptor antagonists,U-opioid receptor agonists, antispasmodics,probiotics,antibiotics,etc.However,in traditional medicine,TCM uses its own unique theoretical basis for the clinical treatment of IBS-D efficacy advantages gradually obvious,theory combined with clinical syndrome differentiation is the fundamental guarantee of its good efficacy.Clinically,most scholars have proved the effectiveness of Sishen pill in the treatment of IBS-D of spleen-kidney yang deficiency type.Objective:In this study,we observed the status of mice and predicted the effect of Sishen pill on the serum levels of VIP,MOT,SP and 5-HT in rats with spleen-kidney yang deficiency syndrome of diarrhea-predominant irritable bowel syndrome.In order to further guide the information and basis of clinical experiments,we hope that this study can increase the experimental basis of traditional Chinese medicine,and use it to guide the study of new drugs for these four types of serum and better play the role of traditional Chinese medicine.Method:Twenty-four 8-week-old rats were randomly divided into four groups:normal group,model group,model+Sishen pill group and model+Desutide group.After the model was successfully established,the normal group and the model group were intragastrically administered with normal saline,the model+Sishen pill group was intragastrically administered with Sishen pill at a dose of 1.83 g/kg by suspension,and the model+Desutide group was administered with Desutide aqueous solution at a dose of 15.23mg/kg.At the end of administration,all rats were sacrificed,eyeballs were collected for blood sampling,serum was separated,and serum SP,VIP,MTL,and 5-HT levels were measured by ELISA.Results:Compared with the control group,the levels of SP,VIP,MTL and 5-HT in the model group were significantly increased（P<0.01）;compared with the model group,the levels of SP,VIP,MTL and 5-HT in the model+TCM group and the model+Western medicine group were significantly decreased（P<0.01）;compared with the model+TCM group,the levels of MTL（Motilin）in the model+Western medicine group were significantly increased（P<0.01）,and the levels of SP,VIP and 5-HT did not change significantly（P>0.05）.Conclusion:1.The serum levels of VIP,MOT,SP and 5-HT in mice after model establishment were significantly increased,which means that these four serological markers may have an effect on the pathogenesis of diarrhea-predominant irritable bowel syndrome,and the increased content easily stimulates the enhancement of gastrointestinal motility.2.Both Sishen pill and Desutide can reduce VIP,MOT,SP and 5-HT in rat serum,indicating that they can relieve diarrhea-predominant irritable bowel syndrome.3.Sishen pill is superior to Desutide in the effect of serum MOT,and the MOT of Desutide group is significantly higher than that of Sishen pill group,perhaps meaning that Sishen pill in the traditional Chinese medicine group has a better therapeutic effect on diarrhea-predominant irritable bowel syndrome compared with Desutide in the western medicine group.