Immunological And Genetic Study Of A Case Of Disseminated Cryptococcosis

Objective: Through immunological and genetic research,this paper explores the causes of a patient with disseminated cryptococcus frequently infected with fungi and viruses in recent years,and identifies the infected cryptococcus strains,so as to find out the possible pathogenic causes and provide new ideas for the treatment of the patient and avoid re-infection.Methods: The clinical data including medical record,blood samples,tissue samples from skin lesions were collected,and the blood samples of the patient were respectively subjected to enzyme-linked immunosorbent assay(ELISA),transcription group second-generation sequencing,and the obtained strains were cultured and identified,so as to explore the reasons why the patient frequently suffered from immune and infectious diseases in recent years.Results: 1.Results of strain culture: the colonies were creamy,shiny and mucoid with smooth edges.2.Strain identification results: BLAST showed that the sequence of the sample amplified by fungal universal primers was 100% similar to that of the Cryptococcus neoformans var.grubii.3.Immunological tests showed that the patient was positive for anti-IFN-γ autoantibodies and negative for anti-GM-CSF autoantibodies.4.The expression of some common genes associated with viral and fungal infection was analyzed by whole gene transcriptome: CARD9,STAT1,DDX20 and other genes were normal expression,and MBL2 gene expression was absent.Conclusion: 1.The patient was infected with Cryptococcus Neoformans Var.Grubii.2.The patient may have anti-IFN-γadult immunodeficiency syndrome.3.Some common genes related to fungal and viral infection such as CARD9,STAT1 and DDX20 can be expressed normally in the patient.The loss of MBL2 gene expression may be related to the infection of Cryptococcus neoformans var.grubii.