Expression Of PATL1 In Gastric Cancer Tissue And Its Effect On Biological Functions Of Gastric Cancer Cells

Study background Gastric cancer（Gastric Cancer GC）is a common clinical malignancy that originates from the epithelial cells of the gastric mucosa.The incidence of gastric cancer is positively correlated with the age of patients,and the ratio of male to female patients is about 2:1^[1];the pathogenesis of gastric cancer is closely related to the regulation of exosomal mi RNAs,which regulate mitogen-activated protein kinase1（MAPK1）,cytokinin kinase6(cyclin-PATL1 has been sequenced and identified as one of the molecules highly expressed in gastric cancer tissues.According to the latest statistics of the World Health Organization（WHO）in 2018,the incidence and death rate of gastric cancer ranked fifth and third in the global malignant tumors,respectively,and the data showed that the number of gastric cancer cases and deaths in China accounted for about 40%of the total number of gastric cancer deaths worldwide^[1].Objective To detect the expression of PATL1 in gastric cancer and explore the relationship between analysis and clinical prognosis of gastric cancer patients;to explore the effect of PATL1 on the proliferation and migration of gastric cancer cells.Methods Combined with TCGA database and HPA database to analyze the expression of PATL1 in pan and gastric cancer,combined with Kaplan-Meier Plotter curve prognosis to analyze the expression of PATL1 in different types of gastric cancer.Forty-two patients with gastric cancer surgery were selected,and the surgically resected gastric cancer tissues and paired paraneoplastic tissues were taken,and the expression of PATL1 in gastric cancer tissues was analyzed by q RCR.The effect of PATL1 on the proliferation of gastric cancer cell line AGS was analyzed by CCK8assay,and the effect of PATL1 on AGS migration was investigated by Transwell and scratch healing assay.westernblotting was performed to detect C-MYC and ATG7protein expression changes after knockdown of PATL1.Expression changes.The results combined with TCGA database using sento（https://www.xiantao.love/products）yielded 18 high expression of PATL1 in 33 pan cancers（P<0.01）and high expression of PATL1 in gastric cancer（P<0.01）Kaplan-Meier Plotter plot prognostic analysis that PATL1 was highly expressed in patients with intestinal-type gastric cancer（P<0.01）.PATL1 expression was higher in gastric cancer patients with short overall survival time than in those with long overall survival time（P<0.01）.PATL1expression was not statistically significant in patients with diffuse and mixed gastric cancer.In combination with the HPA database,PATL1 expression was higher in gastric cancer tissues than in normal gastric tissues.Validation of 42 gastric cancer patient tissues by q PCR showed that PATL1 expression was significantly higher in gastric cancer tissues than in paired paracancerous tissues（P<0.01）.After knockdown of PATL1 in gastric cancer cell line AGS,CCK8 proliferation assay showed that the proliferation ability of gastric cancer cell line AGS was weaker than the control group after knockdown of PATL1（P<0.01）;scratch healing assay and Transwell assay showed that the migration ability of gastric cancer cell line AGS was weaker than the control group after knockdown of PATL1（P<0.01）.In Western Blot assay,knockdown of PATL1 was followed by a decrease in C-MYC and ATG7 protein expression.This suggests that PATL1,C-MYC,and ATG7 may crosstalk each other to regulate autophagy and thus promote the progression of gastric cancer（P<0.01）.Conclusion:PATL1 expression is increased in gastric cancer and correlated with poor prognosis,PATL1 promotes the proliferation and migration of gastric cancer cells,and PATL1has the potential to be a new target for gastric cancer therapy.