Study Of LncRNA Expression Changes In Patients With Systemic Lupus Erythematosus And Their Correlation With Th2 Cells

Objective:By studying the changes in LncRNA expression in patients with systemic lupus erythematosus(SLE),the relationship between the differentially expressed LncRNAs and the number of Th2 cells in patients was preliminarily explored,and the clinical conditions of patients with systemic lupus erythematosus were further analysed.The correlation between performance and Th2 cell-related LncRNA can be predicted to predict the possible mechanism of LncRNA regulating Th2 cell differentiation and development,and provide new perspectives and methods for the diagnosis and treatment of systemic lupus erythematosus.Methods:1.To collect 9 patients with systemic lupus erythematosus in the active stage(SLEDAI>9)as the research objects of this experiment,and to collect the laboratory indicators(C3,C4,ESR、IL-4、IL-6 and IL-10)of SLE patients at the same time,and to calculate the SLEDAI score to evaluate the SLE patients.Disease activity;9 healthy volunteers of the same age and their general information were collected at the same time.These patients were all hospitalised in our department from July 2020 to December 2020,and the volunteers served as the healthy control group.2.The number of Th2 cells in peripheral blood was determined by flow cytometry.By extracting mononuclear cells(PBMC),using the whole transcriptome to sequence the monocytes in peripheral venous blood,and then analysing the expression profile of LncRNA,9 healthy volunteers served as the healthy control group,and the SLE group was excluded,and obtained the relevant differentially expressed LncRNAs and activity indicators of SLE patients through comparative analysis,namely SLEDAI score,ESR,C3,C4,and clinical data such as cytokines,and then used Targetscan and Star Base databases and co-expression network to predict the target genes that Th2 cell-related lnc RNAs may act on.Results:Compared to healthy controls,SLE patients had 240 differentially expressed LncRNAs,including 134 highly expressed LncRNAs and 106 low expressed LncRNAs.Among these differentially expressed LncRNAs,C2CD4D-AS1,AL450352.1,ANKRD44-AS1,AP001363.2,LINC02824,LINC02328,AP001610.2,FAM198BAS1,AP000640.1,LINC00482,PANK2-AS1,MIR503 HG The number of Th2 cells showed a negative correlation,and among the 12 LncRNAs related to Th2 cells,AP001610.2 was positively correlated with C3 serum concentration(P<0.05),and LINC00482,PANK2-AS1,MIR503HG(P<0.05),PANK2-AS1 were negatively correlated with IL-6 serum concentration(P<0.05).Among the above-mentioned lnc RNAs related to the number of Th2 cells,LINC00482,ANKRD44-AS1 and AP000640.1 may regulate the expression of GATA3,PARP14 and LGALS9 through competitive binding to miRNA or through a trans-regulatory mechanism,and further affect the differentiation of Th2 cell development.Conclusion:Compared with healthy controls,the LncRNA expression profiles in SLE patients are different,and some differentially expressed LncRNAs are associated with abnormal changes in Th2 cell counts in SLE patients,and LncRNAs related to the number of Th2 cells are associated with disease activity in SLE patients.Certain associations,and these associations may affect the expression of GATA3,PARP14,and LGALS9 through competitive binding to miRNA or trans-regulation mechanisms,regulate the differentiation of Th2 cells,and further participate in the pathogenesis and disease progression of SLE.