| Objective:To discover the healing effect of EGCG chitosan primarily based gel photosensitive hydrogel on radiation brought about skin damage(RSIs)rats by way of evaluating exceptional kinds of dressings,and similarly explore its repair mechanism on radiation triggered skin damage.It presents a brand new concept and treatment scheme for the diagnosis of medical radiotherapy and epidermal repair.Methods:(1)Preparation of EGCG chitosan based totally gel photosensitive hydrogel(EGCG-CHI-GEL-MA): prepare chitosan(CHI)as 1% acetic acid answer,blend it with methacrylic anhydride liquid(MA),and obtain CHI-MA powder after heating,reaction,neutralization,dialysis,and freeze drying.Dissolve gelatin(GEL)in double distilled water at 50 ℃,and at the equal time dissolve MA in GEL answer at the velocity of0.5ml/min.Stir continuously until the attention is 6%.Add double distilled water at 50 ℃for dilution,and freeze dry to GEL-MA powder.CHI-MA and GEL-MA powders had been prepared right into a positive concentration of aqueous solution,wherein a certain attention of EGCG and 0.2% of LAP answer have been added to provoke with 405 nm light,thus curing to achieve EGCG-CHI-GEL-MA.(2)The molecular mechanism of EGCG chitosan based gel photosensitive hydrogel in the remedy of radiation prompted skin injury: after the status quo of an acute radiation precipitated skin harm version,they had been randomly and averagely divided into EGCG-CHI-GEL-MA treatment institution,CHI-GEL-MA remedy group,untreated radiation institution and normal manage institution.The non-treatment irradiation organization and the everyday manage institution have been handled with the same volume of everyday saline.The EGCG-CHI-GEL-MA treatment group and the CHI-GEL-MA remedy organization had been handled with a single layer of gauze soaked with 0.4 umol/m L of EGCG-CHI-GEL-MA and CHI-GEL-MA for external application.Record the wound restoration time and the radiation pores and skin harm score,and on the equal time,the wound tissue cells had been stained with Sirius red,and the morphology of the wound tissue cells was located in pathology.Then,the expression of CD11 b related protein turned into detected with the aid of immunohistochemistry,and the tumor necrosis thing become detected via enzyme-linked immune response-α(TNF-α)、 Interleukin-1α(IL-1α)And the expression of prostaglandin E2(PGE2)and other proteins.Results:(1)The wound restoration time of EGCG-CHI-GEL-MA institution and CHI-GEL-MA group changed into shorter than that of non-remedy irradiation institution,and the wound recovery time of EGCG-CHI-GEL-MA group became appreciably shorter than that of CHI-GEL-MA organization(P<0.05);(2)Compared with the non-treatment radiation organization,the rats inside the everyday manage organization were extra at level 0 and much less at level IV on the radiation harm score;EGCG-CHI-GEL-MA group had less grade IV(P<0.05),and there has been no extensive difference within the pores and skin injury score among CHI-GEL-MA institution and non-treatment radiation organization.(3)The expression of CD11 b protein in EGCG-CHI-GEL-MA group,CHI-GEL-MA group and non-treatment irradiation institution changed into better than that in everyday manage group(P<0.05),even as the expression of CD11 b protein in EGCG-CHI-GEL-MA institution and CHI-GEL-MA institution became lower than that during non-treatment irradiation institution(P<0.05);The expression of CD11 b protein in EGCG-CHI-GEL-MA institution turned into lower than that in CHI-GEL-MA organization(P<0.05);(4)In addition,TNF in non-treatment irradiation organization,CHI-GEL-MA institution and EGCG-CHI-GEL-MA institution-α、 IL-1 α The expression of PGE2 and PGE2 protein turned into higher than that of regular organization(P<0.05),and TNF in EGCG-CHI-GEL-MA institution and CHI-GEL-MA institution-α、 IL-1α The expression of PGE2 protein was decrease than that of non-remedy irradiation institution(P<0.05);TNF in EGCG-CHI-GEL-MA organization-α、 IL-1α The expression of PGE2 protein became decrease than that of CHI-GEL-MA organization(P<0.05).Conclusion:(1)Both EGCG-Chitosan gelatin photosensitive hydrogels and traditional chitosan gelatin hydrogels can treat radioactive skin injury,and EGCG-chitosan gelatin photosensitive hydrogels have better therapeutic effect than traditional chitosan gelatin hydrogels.(2)Egcg-chitosan gelatin photosensitive hydrogel can accelerate the repair and healing of radioactive skin injury,and down-regulate the expression of tumor factors and pro-inflammatory factors. |
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