| The transcranial ultrasound stimulation(TUS)is an emerging treatment for neurological diseases.It has the advantages of non-invasive damage,high penetration depth and high spatial resolution.However,until now,there have been few clinical cases of TUS being applied.Therefore,in order to further study the potential value of TUS in its application and reveal the key scientific question of whether TUS has preventive and therapeutic effects on migraine,this thesis used low-intensity TUS to treat migraine model rats,and recorded the changes of cortical cerebral blood flow velocity and head-scratching behavior of rats throughout the experiment.The main work of this article consists of the following three parts:(1)A set of micro laser speckle contrast imaging system was independently designed,which weighed only 25 g overall,allowing it to be worn on the head of rats without affecting their free movements.The system can collect the changes of cortical cerebral blood flow velocity of rats in different states in real time.To study rat behavior more comprehensively,we also designed a behavioral recording system to record changes in rat behavior in real time.(2)We studied on the regulatory effect of low-intensity TUS on cerebral blood flow and tail electromyography in normal rat cortex.Taking freely moving rats as the research object,ultrasound with different parameters was used to stimulate the brain tissues of eating rats and sleeping rats,and the cerebral blood flow velocity and tail electromyography of rats in the cortex were recorded to analyze the regulatory effect of low-intensity TUS on brain tissue.The effectiveness of ultrasound on cerebral blood flow velocity and tail electromyography regulation in rat cerebral cortex was demonstrated.(3)We study on the preventive and therapeutic effects of low-intensity TUS on migraine rats.Changes in cortical cerebral blood flow velocity and head-scratching behavior in 8 groups of rats in the free moving rats were recorded before and after ultrasound stimulation.The difference between ultrasound prevention and ultrasound therapy was observed,and the deaf mouse model was used to exclude the influence of auditory effects.The experimental results proved that low-intensity TUS could inhibit the onset of migraine in rats,which provided a research method for ultrasound treatment of migraine and promoted the application of ultrasound stimulation in the clinical treatment of migraine.The above research results reveal that low-intensity TUS has a regulatory effect on cortical cerebral blood flow,tail electromyography and head-scratching behavior in rats,providing a new idea for the treatment of migraine. |
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