Design And Test Of Intelligent Portable Electrochemical Workstation For Diabetes Index Detection

Insulin and blood glucose concentrations in the human body are important parameters for the early assessment and diagnosis of diabetes,and the coupled measurement of the two substances is of great value for issues such as diabetes monitoring.At present,insulin or glucose testing based on electrochemical principles relies on commercial electrochemical workstations to complete,which has problems such as high cost and complicated operation.Therefore,there is a broad application prospect to design a low-cost,portable and intelligent electrochemical testing device that can realize the combined measurement of insulin and glucose.To address the above problems,circuit design and analysis were carried out based on electrochemical theory,and a linear regression algorithm was used to construct an intelligent and portable electrochemical workstation with the advantages of low cost,easy operation and simultaneous measurement of insulin and glucose under the same sample system,etc.The developed electrochemical workstation was then tested systematically.The specific work is as follows:(1)The principle of electrochemical sensors was analysed to dig deeper into the detection of insulin and blood glucose.An electrochemical detection platform was built,the constant potential and signal generator circuits used to generate current in the threeelectrode reaction system were designed,and the range of microcurrents generated by the detection circuit was analysed.The completed hardware system is designed to be smaller than a bank card and costs less than $100.The materials required to prepare the experiments were then determined,the screen-printed electrodes were modified using electrodeposition techniques,and the modified electrodes were tested by cyclic voltammetry experiments,which demonstrated that the modified screen-printed electrodes have excellent electrical activity and can be further applied to the quantitative detection of insulin and blood glucose.(2)A measurement protocol for insulin and glucose was developed,and the solution concentrations of insulin and glucose were determined by cyclic voltammetry based on the electrochemical principles from the physicochemical properties and biological structure of the measured substances.It has been shown experimentally that the detection of different concentrations of insulin and glucose solutions can be achieved within a small margin of error by means of the electrochemical detection principle and modified electrodes.The sensitivities of insulin and glucose were 48 μA/mmol and 15.82 μA/μmol,respectively.(3)Based on the large amount of experimental data measured by cyclic voltammetry,features were extracted and data sets were constructed.On this basis,the concentrations of the mixed insulin and glucose solutions were predicted by a linear regression classification method.The average deviation of the predicted insulin solution concentration was 6.52%,and the average deviation of the predicted glucose solution concentration was 4.36%.The portable workstation measurement based on electrochemical and machine learning principles proposed in this paper,with classification algorithms written directly into a small workstation,has the advantages of portability,low cost and high accuracy,and has a greater prospect of application in clinical diagnosis,experimental measurement and home monitoring of diabetes.