| Objective: To establish a mouse model of rotator cuff injury,and observe the supraspinatus muscle-humerus tendon-bone junction after external application of chicken embryos for modeling by histology,biomechanics,biochemistry and enzyme-linked immune sorbent assay(ELISA),to evaluate the effectiveness of the external application of chicken embryo therapy on the healing of rotator cuff tendon bone in mice and to elucidate part of the mechanism.Methods: 39 C57 mice,11 in the blank group and the remaining 28 were moulded.22 mice were selected after 1 week of successful moulding,free from infection and did not die,and were randomly divided into experimental group(chicken embryo eggs applied externally)and control group,11 mice in each group.Five mice in the experimental and control groups were randomly selected for HE staining and light microscopic observation of the healing of supraspinatus tendon bone stop at 1 and 3weeks of intervention;the ultimate tensile load at the tendon bone junction was measured by biomechanical test in the control and experimental groups at 1 and 3 weeks of intervention;the blood calcium and alkaline phosphatase levels were observed by biochemical analyzer in the control and experimental groups at 1 and 3 weeks of intervention.The data were analysed by independent sample t-test.Network pharmacology was used to explore the biological process and signaling pathway of chicken embryos on tendon-bone junction.Interleukin(IL)-6 in joint nodes was selected,and the concentration of IL-6 in blank group,control group and experimental group for 1 and 3 weeks of intervention was analyzed by single factor variance analysis.Results: Biomechanics: At 1 week of intervention,the ultimate tensile load of the control group was not statistically significant compared to that of the experimental group(P>0.05),and at 3 weeks of intervention,the ultimate tensile load of the control group was higher than that of the experimental group(P<0.05).Biochemistry: The blood calcium and alkaline phosphatase concentrations in the experimental group were not statistically significant compared to those in the control group at 1 week of intervention(P > 0.05),and at 3 weeks of intervention,the blood calcium and alkaline phosphatase concentrations in the experimental group were higher than those in the control group(P < 0.05).Histology: At 1week of intervention,some fibroblasts and other tissue cells without sequence were visible at the tendon bone stops in the experimental and control groups.At 3 weeks of intervention,fibroblasts were seen at the tendon bone stops in the control group,and the fibroblasts at the tendon bone stops in the experimental group were proliferating,and the proliferating fibroblasts were densely arranged,and large foci of calcification were visible.ELISA: At 1 week of intervention,the IL-6concentration was statistically significant(P<0.05)between the blank group,the control group and the experimental group,with the experimental group being higher than the control group and the control group being higher than the blank group.There was no statistically significant difference in IL-6 concentration between the blank group,control group and experimental group after 3 weeks of intervention(P>0.05).Conclusion: External application of chicken embryo(14 days)promoted tendon bone healing in a rotator cuff injury model.Chicken embryo(14days)external application increased IL-6 concentration around the tendon-bone attachment point of rotator cuff injury in the early stage,and decreased IL-6 concentration as the tendon-bone healed. |
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