The Expression Of LncRNA PPP1R14B-AS1 In Bladder Cancer Tissues And Its Effect On Biological Behaviors Of Bladder Cancer Cell Proliferation,Migration And Invasion

Part Ⅰ Expression of PPP1R14B-AS1 in bladder cancer cell line and bladder cancer tissueObjective:To explore the effects of overexpression of PPP1R14B-AS1 gene and knockdown of PPP1R14B-AS1 gene on the biological behavior of bladder cancer cell proliferation,migration and invasion.Methods:Real-time quantitative fluorescent PCR(q RT-PCR)was used to detect the expression of PPP1R14B-AS1 gene in 2 strains of bladder cancer cells(5637,T24)and to compare the expression of PPP1R14B-AS1 gene in42 cases of bladder cancer tissues with that in control normal bladder tissues.Results:1.Expression of PPP1R14B-AS1 gene in bladder cancer cell lines: The relative expression level of PPP1R14B-AS1 gene was significantly increased in two bladder cancer cell lines(5637 and T24),and the expression level of PPP1R14B-AS1 was the highest in T24 cell lines.2.The expression of PPP1R14B-AS1 gene in BC tissues and normal tissues: The relative expression level of PPP1R14B-AS1 in BC tissues was significantly higher than that in normal bladder tissues of the control group.The expression level of PPP1R14B-AS1 in BC tissues was correlated with tumor stage(T stage),but not with tumor size,age,history of smoking,or history of hypertension.Conclusions:1.PPP1R14B-AS1 gene is highly expressed in bladder cancer cell lines,suggesting that PPP1R14B-AS1 gene may play a role in the occurrence and development of bladder cancer.2.The expression of PPP1R14B-AS1 gene in BC tissues was significantly higher than that in its corresponding normal tissues,and was correlated with the tumor staging in BC tissues.Regardless of tumor size,age,or history of smoking or hypertension.Part Two: The effect of PPP1R14B-AS1 on biological behaviors of bladder cancer cell proliferation,migration and invasion.Objective: To investigate the effects of overexpression of PPP1R14B-AS1 gene and knockdown of PPP1R14B-AS1 gene on the biological behavior of bladder cancer cell proliferation,migration and invasion.Methods: 1.Overexpression vector pc DNA3.1-PPP1R14B-AS1 and knockdown vector si-PPP1R14B-AS1 were constructed to transfect bladder cancer cells 5637 and T24,respectively,and the transfection efficiency was verified by RT-q PCR method.2.The proliferation ability of bladder cancer cells after overexpression of PPP1R14B-AS1 gene and knockdown of PPP1R14B-AS1 gene was observed by MTS and cloning experiments.3.The migration ability of bladder cancer cells after overexpression of PPP1R14B-AS1 gene and knockdown of PPP1RB-AS1 gene was observed by scratch test.4.Transwell assay was used to observe the changes in bladder cancer cell invasion ability after overexpression of PPP1R14B-AS1 gene and knockdown of PPP1R14B-AS1 gene.Results:1.Construction of overexpression vector pc DNA3.1-PPP1R14B-AS1 and knockdown vector pc DNA3.1-PPP1R14B-AS1: Overexpression vector pc DNA3.1-PPP1R14B-AS1 was successfully constructed and transfected into bladder cancer cell 5637,and knockdown vector pc DNA3.1-PPP1R14B-AS1 was transfected into bladder cancer cell T24.Verification of transfection efficiency: The RT-q PCR method was used to verify the transfection efficiency.It was found that the relative expression levels of the overexpression group and the knockdown group were significantly different from those of the control group,with statistical significance.2.Overexpression of PPP1R14B-AS1 gene can significantly enhance the proliferation,migration and invasion of bladder cancer cells.3.Knockdown of PPP1R14B-AS1 gene can significantly inhibit the proliferation,migration and invasion of bladder cancer cells.Conclusions:Overexpression of PPP1R14B-AS1 gene can enhance t he proliferation,migration and invasion of bladder cancer cells.Knockd own of PPP1R14B-AS1 gene can inhibit its proliferation,migration and invasion.