Effect Of Aggregates Of Smooth Endoplasmic Reticulum In Oocytes On Clinical Outcomes Of Human Assisted Reproduction

In order to analyze the development potential and clinical outcomes of smooth endoplasmic reticulum aggregate(SERa)oocyte-derived embryos,and explore the formation and the usability of SERa oocyte.The data came from patients with in vitro fertilization(IVF)or intracytoplasmic sperm injection(ICSI)at the Chengdu xinan gynecological hospital and Jinjiang district maternal and child health hospital from January2019 to December 2021.After sorting and screening,the data was divided into the ICSI group and the IVF group,and the following comparisons were made:(1)According to whether the oocytes in the cycle showed SERa,the groups were divided(SERa+ cycle group and SERa-cycle group),and the patient’s basic situation,embryonic development potential,and clinical outcomes were compared.(2)Compare the effects of the number,size,and disappearance time of different SERa in oocytes on embryonic development.(3)Set different classification criteria based on the proportion of SERa oocytes present in a single SERa+ cycle of oocytes,the proportion of repeated SERa+ cycles in the same patient,and the source of transferred embryos,and compare their patient’s basic situation,embryonic development potential,and clinical outcomes.The research results are as follows:(1)ICSI group:(1)Patients in the SERa+ cycle group have lower age,significant differences in some sex hormone levels,and longer medication duration(P<0.05);The normal fertilization rate and blastocyst formation rate were significantly higher,while the cleavage rate of excellent embryos was significantly lower(P<0.05);SERa reduced the implantation rate and multiple pregnancy rate of fresh embryos(P<0.05).(2)When the number of SERa is small,its cleavage time into 3-4 cells is longer(P<0.05);When the size of SERa is larger,its normal fertilization rate is higher(P<0.05);When SERa exists for a longer time,the normal fertilization rate is lower,and the pronuclear recession time and cleavage time into 2 cells are longer(P<0.05).(3)In the same SERa+ cycle,patients in the low proportion of SERa oocytes group were significantly younger in age,with significantly higher numbers of retrieved oocytes,normal fertilization rates,and high-quality blastocyst formation rates(P<0.05);Compared to the mixed transplantation group or the transplantation of normal oocytes in the same cycle,there was no significant difference in the abnormality rate of the SERa+ oocytes group(P>0.05).(2)IVF group:(1)Patients in the SERa+ cycle group have lower age,significant differences in some sex hormone levels,higher medication dosage,and longer medication duration(P<0.05);The normal fertilization rate and cleavage embryo rate were significantly lower,while the blastocyst formation rate was significantly higher(P<0.05);SERa increased the implantation rate of frozen embryos(P<0.05).(2)The blastocyst formation rate of largesized SERa is higher than that of small-sized SERa,and the time for cleavage into 3 cells is also longer(P<0.05).(3)The group with a low proportion of SERa+ oocytes had significantly lower age,significantly higher number of retrieved oocytes,normal fertilization rate,and blastocyst formation rate(P<0.05);There was no significant difference(P>0.05)in the deformity rate between the SERa+ oocytes transplantation group and the mixed transplantation group or the same cycle SERa-oocytes transplantation group.The above results indicate that the formation of SERa in oocytes is influenced by various factors such as patient age,medication dosage,and medication duration.The number,size,and disappearance time of SERa can affect embryonic development.The appearance of SERa can affect the rate of excellent embryo cleavage,but it will not affect the malformation rate of newborns.