Study On The Correlation Between Epstein-Barr Virus DNA Copy Number Scale And Kawasaki Disease

Background and purposeKawasaki disease(Kawasaki disease,KD),also known as mucocutaneous lymph node syndrome,is more common in children under 5 years of age.It often causes multiple organ damage,especially cardiovascular system damage.It is the primary cause of acquired heart disease in children.In children with KD who have not received effective clinical intervention,the rate of CAL can be as high as 20%～25% of children with KD who receive timely and effective clinical interventions still maintain a rate of 3% to 5% of CAL.A small number of children can even develop coronary artery stenosis or myocardial infarction,which is life-threatening.Therefore,it is particularly important to clarify the cause of KD and to reduce the occurrence of KD and the harm of coronary artery lesions(CAL)to children through effective treatment.At present,the cause of KD is still unclear.Studies have found that infection factors,genetic susceptibility,environmental factors,and immune abnormal activation may all be greatly related to the occurrence of KD.Since the outbreak of new coronavirus infection at the end of 2019,some children with new coronavirus infection have developed symptoms and signs similar to KD,which has attracted the attention of pediatricians at home and abroad.It shows that infectious factors may be involved in the occurrence of KD.Epstein-Barr virus(EBV)is widely present in nature and can cause a variety of human diseases.The common features of EBV infection and KD include epidemiology,prevalence age,symptoms and signs,etc.,to explore whether EBV infection Participating in the occurrence of KD and other possible causes of KD are the current research hotspots.EBV-DNA copy number can reflect the active degree of EBV replication.Studies have found that the clinical manifestations and therapeutic effects of childhood leukemia,nasopharyngeal carcinoma and other diseases will be affected by EBV-DNA copy number,but so far there is no EBV-DNA copy.Research on the correlation between number and KD.This topic compares the EBV positive rate of the KD group and the control group,and compares the general conditions,clinical manifestations,laboratory tests,and the incidence of CAL in the EBV positive group and the EBV negative group,and compares the high EBV-DNA copy array and the low EBV-DNA The general condition of the copy array,clinical manifestations,laboratory tests,and the incidence of CAL,to explore whether EBV is related to the occurrence of KD,whether EBV infection will affect the clinical manifestations of KD,laboratory tests,CAL It has an impact on the incidence of KD,and explores whether the occurrence of KD is related to EB virus infection,and provides guidance for clinical work.MethodsSeptember 2018 to December 2020,Sixty-six case worn by 0 to 5 years old who were diagnosed and admitted to pediatric internal medicine department of the Second Affiliated Hospital of Harbin Medical University were selected as the research objects.During the same period,35 healthy case worn by 0 to 5 years old who were treated in the Outpatient Department of Pediatrics of our hospital were selected as the control group.All children in the control group received venous blood at the outpatient clinic on the day of treatment.All children in the KD group received venous blood on an empty stomach in the morning of the next day and underwent cardiac color Doppler ultrasound.Moreover,children with KD need to review cardiac color Doppler ultrasound at the first,third and sixth months after discharge.The positive rates of EBV in control group and KD group were compared.Based on the EBV test results,the children,who in the KD group were parcelled out into EBV-positive group and EBV-negative group.Based on the median EBV-DNA copy number,children in the EBV-positive group were parcelled out into high EBV-DNA copy array and low EBV-DNA copy array.Compare the EBV-positive group and the EBV-negative group,the high EBV-DNA copy array and the low EBV-DNA copy array in gender,age,length of hospitalization,fever time,incidence of rash,incidence of cervical lymphadenopathy,incidence of conjunctival hyperemia,respectively Whether there are statistically significant differences in the rate of white blood cell(WBC)count,percentage of neutrophils,platelet count(PLT)count,C-reactive protein(CRP)and the incidence of CAL.Observe whether the data are normally distributed,use the mean for the data describing the normal distribution,and use the median for the data describing the non-normal distribution.Normal The distributed data uses the t test,and the non-normally distributed data uses the rank sum test.Counting data in terms of case number(percentage),defferences among groups were compared with the chi-square test.there is a significant differance when P<0.05.Results1.Compared with the control group,the KD group had higher positive rate of EBV.he difference was statistically significant(P<0.05)2.Compared with the EBV-negative group,the EBV-positive group had older age of onset,longer fever time,higher incidence of CAL,lower incidence of bulbar conjunctival hyperemia,and lower PLT.The difference was statistically significant(P<0.05).3.High EBV-DNA copy array is lower.EBV-DNA copy array has long hospital stay,high CRP,low incidence of rash,low PLT,and the difference is statistically significant(P<0.05).Conclusions1.Children with KD have a high EBV infection rate,and EBV infection may be one of the pathogenic factors of Kawasaki disease.2.Compared with the EBV-negative group,the EBV-positive group has older age,longer fever time,higher incidence of CAL,lower incidence of bulbar conjunctival hyperemia,and lower PLT.3.Compared with the high EBV-DNA copy array and the low EBV-DNA copy array,the length of hospital stay is long,CRP is high,the incidence of skin rash is low,and PLT is low.