Correlation Between Intestinal Flora And Their GLP-1 Levels In New-onset Type 2 Diabetic Patients With Food Addiction

Purpose To study the characteristics of gut microbiota and the association with endogenous GLP-1 levels in patients with new-onset type 2 diabetes mellitus with food addiction,and to provide new diagnostic ideas for new-onset type 2 diabetes mellitus with food addiction.Methods From August 2020 to December 2021,13 patients with type 2 diabetes mellitus suffering from food addiction were recruited as subjects according to the inclusion and exclusion criteria,and 13 patients with type 2 diabetes mellitus without food addiction were selected as controls.The association with their endogenous GLP-1levels was analyzed.The general clinical data of the patients were collected,and the blood and fecal samples of the patients were collected according to the standard procedure,various biochemical indicators in the serum were detected,and the characteristics of clinical parameters among the groups were analyzed.Relevant bioinformatics techniques were used to compare the abundance and diversity of the flora among the groups,and to analyze the correlation between the gut microbiota and the clinical parameters of each group.Results(1)Clinical index analysis: △TGLP-1 was significantly higher and statistically significant in subjects in the high sugar and high fat addiction groups compared with the control group(P < 0.05);(2)Gut microbiota analysis: no significant differences were found in the flora diversity of the three groups of subjects with combined high fat addiction,high sugar addiction and simple new onset type 2 diabetes without addiction;screening for differential species revealed that patients with combined high fat food addiction The abundance of butyric acid-producing bacteria such as Vibrio butyricus,Eubacterium butyricum,Eubacterium spp.and Fusicatenibacter was decreased in patients with combined high sugar addiction,and a significant decrease in the abundance of Butyricoccus and Fusicatenibacter was observed in patients with combined high sugar addiction.(3)Correlation analysis between intestinal flora and clinical indicators: it was found that the level of recent mean blood glucose was significantly and positively correlated with Clostridium_IV,Romboutsia and Atopobium;BMI was significantly and positively correlated with Allisonella and negatively correlated with Eubacterium.It was found that endogenous GLP-1 levels were positively correlated with the relative abundance of Anaerostipes and Faecalibacterium.Conclusions(1)ΔTGLP-1 levels were significantly higher in patients with combined food addiction compared with new-onset 2 new diabetes alone,suggesting the possibility of increased sensitivity of intestinal L-cells to nutrients in the intestinal lumen in this group of patients;(2)analysis of gut microbiota showed that,compared with subjects with type 2 diabetes alone,patients with combined high-fat food addiction had reduced abundance of certain butyric acid-producing bacteria,and combined high-sugar food addiction patients showed a significant decrease in related conditionally pathogenic bacteria.This alteration may lead to weakening of the intestinal barrier,which ultimately leads to disruption of the homeostasis of the gut microbiota and aggravation of the inflammatory state;(3)correlation analysis between clinical indicators and gut microbiota in subjects revealed that clinical indicators related to the development of type 2 diabetes,such as GA,Hb A1 c,BMI and GLP-1,were closely related to the abundance of specific flora.However,the elevation of ΔTGLP-1 in patients with combined food addiction may not be directly caused by changes in the gut microbiota.