Effect Of Compound Chamomile And Lidocaine Hydrochloride Gel On Soft Tissue Healing In SD Rats Tooth Extraction Wounds

Objective: By Exploring the effect of compound chamomile and lidocaine hydrochloride gel on soft tissue healing in SD rats tooth extraction wounds,to provide a feasibility basis for the clinical application of the gel to promote soft tissue healing by tooth extraction.Methods: 1.Tooth extraction wounds of the left and right bilateral mandibular third molars of 20 SD female rats(age: 6-7 weeks)were to establish a model of wound healing.2.The left tooth extraction was used as the experimental group,the compound chamomile lidocaine gel was applied on the surface of that after hemostasis.The right tooth extraction was the control group,and no special treatment was done after hemostasis,let it heal naturally.The left and right tooth extraction wound was washed by the normal saline twice a day(at 8:00 and 20:00,respectively)after surgery.The compound chamomile lidocaine gel was applied on the extraction surface on the experimental side and the control side was rinsed only.3.The tooth extraction photos and gingival soft tissue samples were collected after separating the mandible after 5 rats sacrifice on the 3rd,7th,10 th and 14 th days after the operation.4.The residual areas of tooth extraction wounds were calculated using Image-Pro Plus(IPP)6.0,and performed a statistical analysis by using SPSS25.0 to compare whether the two groups were statistically different.HE staining was used to observe the morphology of two groups of gingival tissues at different times and analyzed the number of blood capillary on sections by SPSS 25.0,to compare the soft tissue healing differences between the two groups.Results: 1.On the 3rd day after tooth extraction,the residual areas of tooth extraction wounds between the experimental and control groups was not significant differences(P>0.05);the residual areas of tooth extraction wounds in the experimental group on the 7th,10 th and 14 th days after tooth extraction was significantly smaller than that in the control group(P <0.05).Compared to the residual areas of tooth extraction wounds,there were significantly reduced in the periods on days 3rd and 7th,7th and 10 th,10th and14th(P<0.05),within-group comparison of the control and experimental groups.The healing rate of the experimental group was significantly faster from day 3rd to 7th than that of the control group,and the two groups were flat from day 7th.2.HE staining results showed that on the 3rd,7th and 10 th days after surgery,compared with the control group,the experimental group had less inflammatory cell infiltration,more capillary hyperplasia(P<0.05),and more fibroblasts and collagen fiber hyperplasia,and collagen fiber arrangement was more regular.On the 14 th day after surgery,the two groups were basically similar in the number of capillaries and fibroblasts(P>0.05),but the collagen fiber bundles in the experimental group were more thick and more orderly than the control group,and no obvious inflammatory cell infiltration was seen in both groups.Conclusions:1.Chamomile chamomile and lidocaine hydrochloride gel can inhibit the inflammatory response of tooth extraction creation in SD rats,and promotes soft tissue healing in SD rats tooth extraction.2.The results of this experiment suggest that compound chamomile and lidocaine hydrochloride gel may be applied to promote the healing of tooth extraction wounds clinically.