LncRNA SNHG8 Is Involved In The Progression Of Invasive Pituitary Adenoma By Upregulating KMT5A Via CeRNA-mediated MiR-335-5P Evasion

Objective :Differentially expressed lncRNAs were screened from invasive and non-invasive pituitary adenomas,and could be used as competitive endogenous RNA(ceRNA)to regulate miRNAs,so as to explore the effect of significantly up-regulated lncrnas on the proliferation of pituitary adenomas and the molecular mechanism.Methods:1.Invasive pituitary adenoma(IPA)tissue samples and non-invasive pituitary adenoma(NIPA)tissue samples were sequenced using RNA-seq technology to screen out differentially expressed lncRNAs.By constructing ceRNA regulatory network,LncSNHG8-miR335-KMT5 A signal pathway was obtained.2.The expression levels of LncSNHG8 and miR-335-5P in invasive pituitary adenoma(IPA)tissue samples and non-invasive pituitary adenoma(NIPA)tissue samples were detected by Quantitative real-time PCR(q RT-PCR).3.The effects and mechanisms of LncSNHG8 and its ceRNA network on the proliferation of pituitary adenoma(PA)cells were confirmed by gene overexpression or silencing techniques combined with WB,Q-PCR,flow cytometry assay,CCK-8assay,EdU staining,double luciferase reporter gene assay,wound healing assay,transwell migration and invasion in PA cell lines AtT-20 and GT1-1.Results:LncSNHG8 was overexpressed in IPA tissues.Abnormally overexpressed LncSNHG8 can promote cell proliferation,migration and invasion by affecting cell cycle and apoptosis in vitro.In terms of mechanism,overexpressed LncSNHG8 restricts the expression of miR-335-5p and up-regulates the expression of KMT5 A through the ceRNA mechanism,promoting the proliferation of pituitary adenoma cells and participating in the progression of IPA.Conclusion:LncSNHG8 can promote the progression of pituitary adenoma and is a potential therapeutic target for invasive pituitary adenoma.