Study On The Effect And Mechanism Of Panax Notoginseng On Ischemia-reperfusion Injury Of Rat Skin Flap Based On Network Pharmacolog

Objective:To explore the mechanism of action and core targets of Panax ginseng within the treatment of ischemia-reperfusion injury of skin flaps employing a Network pharmacology.Further,the consequences of Panax ginseng on ischemia-reperfusion injury of skin flaps were through an experiment verified.Methods:Through TCMSP,the target genes related to Panax notoginseng were searched,the disease-related targets were got from the four databases of GeneCards,OMIM,PharmGkb and TTD.The target related to Panax notoginseng and FIRI were intersected through Cy to scape 3.8.0 software,the protein interaction network of Panax ginseng for FIRI treatment were analyzed.R language were used to perform GO analysis and KEGG pathway analysis depend on key target genes.The theoretical basis of target and pathway information predicted by network science was used for experimental validation.Forty-five adult healthy SPF-grade rats at 6-8 weeks of age were randomly divided into sham-operated group(Group A),model group(Group B),and panax ginseng treatment group(Group C)with 15 rats in each group.A model of ischemia-reperfusion injury to the left lower abdominal flap of rats was made.At 7 d after surgery,the serum concentrations of TNF-α and IL-6 were measured in rats,and the flap tissues were analyzed by HE staining.Results:1.Network pharmacology showed 8 active components of Panax notoginseng,47 8 FIRI-related target genes,and 60 common target genes of Panax notoginseng and FIRI.2.The mechanism of panax notoginseng assuaging isCHEMIa-reperfusion injury of skin flap mainly involves biological processes such as cell reaction to chemical stress,oxidative stress and reactive oxygen metabolism.3.KEGG pathway enrichment analysis urged that the protective effect of Pantoginseng on ISCHEMIa-reperfusion injury was principally associated with TNF,TLRs,HIF-1,NF-kB,MAPK,estrogen and other signaling pathways.4.HE staining of skin flap 7 days when operation showed that there was no obvious edema and inflammatory cell exudation in sham operation group,while the number of tissue swelling,exudation and inflammatory cell in Panax notoginseng treatment group was less than that in model group(P<0.05).5.The levels of TNF-α and IL-6 in blood of Panax notoginseng treatment group were lower than those of model group on 7 days after operation(P<0.05),and the difference was statistically significant.Conclusion:1.The key targets of Panax notoginseng in the treatment of ischemia-reperfusion injury of skin flap include TNF,AKT1,VEGFA,PTGS2 and IL1B.The mechanism of action mainly involves biological processes such as cell reaction to chemical stress,oxidative stress and reactive oxygen metabolism.It is closely related to TNF,TLRs,HIF-1,NF-kB,MAPK estrogen and other signaling pathways.2.Panax notoginseng can reduce tissue edema and inflammatory factor exudation,reduce serum TNF-α,IL-6 content,inhibit inflammatory reaction.