Strychnine Exerts Anticancer Effects In Osteosarcoma Via The PI3K-AKT Signaling Pathwa

Objective: To explore the effects of brucine on cell proliferation,plate cloning,migration and invasion,cell cycle and apoptosis and further explore some of the molecular mechanisms.Methods:Human osteosarcoma HOS and 143 B cells were cultured in vitro,and the proliferation activity of osteosarcoma cells was detected by3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide(MTT)colorimetry,EDU experiment and cell clone formation experiment.Cell scratch test was used to detect the wound healing ability and lateral transfer ability of cells,and Transwel cell test was used to detect the longitudinal invasion ability of osteosarcoma cells.The cell cycle and apoptosis were detected by flow cytometry,and the mechanism of brucine on osteosarcoma was predicted by introducing bioinformatics prediction technology.Finally,the protein expression was verified by Western blot experiment.Results: Macine time-and dose-dependent inhibited the proliferation of HOS and 143 B cells;also inhibited tumor cell migration and invasion;promoted apoptosis of HOS and 143 B cells by inhibiting PI3K-AKT signaling,and downregulated p-PI3 K,p-AKT,MMP 9,Cyclin D1,and C-caspase9 protein content in HOS and143 B cells.Conclusion: Machine inhibited the proliferation of osteosarcoma HOS and143 B cells,inhibited the invasion and migration ability of osteosarcoma HOS and143 B cells,and promoted the apoptosis of osteosarcoma cells by inhibiting PI3K-AKT signaling;Western blot verified that brucine promoted the apoptosis of HOS and 143 B cells by regulating p-PI3 K,p-AKT,MMP 9,Cyclin D1,and C-caspase9 proteins.