Effect Of Compound Qinbai Granules On CD4~+T Cell Immune Homeostasis In Rats With Ulcerative Coliti

Objective: To confirm the regulative effect of Fufang Qinbai Granule on Th1 / Th2 and Th17 / Treg cell homeostasis in ulcerative colitis(UC)rats,and to explore the therapeutic mechanism of UC.Methods: In this study,the rat model of UC was established by DSS induction.The rats were divided into normal group,model group,metharazine group and Fufang Qinbai granule group.The normal group and the model group were given 0.9% normal saline by gavage,the mesalazine group was given mesalazine solution by gavage,and the Fufang Qin Bai granule group was given Fufang Qin Bai granule solution by gavage.After 3 weeks of intervention,the physiological activity,fecal characters and fecal occult blood were observed.The colonic histopathological changes were detected by H.E.staining method.Thl / Th2,Th17 / Treg cell subsets in spleen and mesenteric lymph node were detected by flow cytometry.RT-PCR was used to detect the m RNA expression of reverse transcription factor T-bet,GATA-3,RORγt,Foxp3 in intestinal tissue of rats.The protein expression of reverse transcription factor RORγt and Foxp3 was measured by Western Blot.To explore the mechanism of Fufang Qinbai Granule in treating UC.Results: The UC rat model was induced by DSS.The weight of rats in the model group was obviously decreased,the eating and drinking decreased,and the symptoms of loose stool and hematochezia appeared.The length of the colon becomes shorter,and the intestinal mucosa is hyperemic and festering.Mesalazine group and Fufang Qinbai granule group improved the symptoms compared with the model group,tended to the normal group,intestinal mucosa and intestinal wall morphology and structure slowly recovered,the number of ulcer lesions decreased,redness and swelling area decreased significantly.The m RNA content of T-bet and RORγt and the protein expression level of RORγt in colon tissue of model rats were significantly increased,while the m RNA content of GATA-3 and Foxp3 and Foxp3 protein expression level were significantly decreased.The percentage of IFN-γ cytokine in mesenteric lymph node and spleen of rats in model group was increased,and that of IL-4 cytokine was decreased.In the mesenteric lymph node and spleen of rats in the model group,the percentage of IL-17 cytokine increased and Foxp3 cytokine decreased.After treatment,m RNA content of T-bet and RORγt and protein expression of RORγt decreased in metharazine group and Fufang Qinbai granule group.The m RNA content of GATA-3 and Foxp3 and the protein expression level of Foxyp3 increased.The percentage of IFN-γ cytokine decreased significantly and that of IL-4 increased significantly in mesenteric lymph node and spleen of rats.The percentage of IL-17 cell factor was significantly decreased,while that of Foxp3 cell factor were significantly increased.Conclusion: 1.Fufang Qinbai Granule can effectively improve the general symptoms and intestinal mucosa of UC rats induced by DSS;2.Fufang Qinbai Granule could inhibit Th1 cell differentiation by down-regulating T-bet transcription factor,and increase Th2 cells differentiation by up-regulating GATA-3 transcription factor.By down-regulating RORγt transcription factor level and up-regulating Foxp3 transcription factor level,inhibiting Th17 cell differentiation,promoting Treg cell differentiation,in order to regulate Th17 / Treg cell balance,to reduce inflammation,enhance immunosuppression,restore intestinal homeostasis of UC treatment effect.