| Objective: To investigate the effect of chaiculongvitt combined with capecitabine on tumor inhibition and expression of TGF-β1,Smad3 and Smad7 in triple negative breast cancer nude mice.Materials and Methods: Forty female BALB/c-nu nude mice were randomly divided into five groups: blank control group,model control group,bupleuron vitexes group,capecitabine group and combination group(bupleuron vitexes + capecitabine group).Eight females were divided into one group,and MDA-MB-231 cells were inoculated in the breast pad of the nude mice in front of the sternum to construct a triple negative breast cancer(TNBC)model.Rats and humans were given the drug according to the body surface area.The model group was given equal dose of normal saline.The gavage period was 21 days.The ocular blood was collected to detect the liver and kidney function indexes in the serum of nude mice,and the tumor bodies of each group were removed and weighed to calculate the tumor inhibition rate.HE staining and Masson staining were used to detect the pathological morphology and fibrosis morphological changes of tumor tissues.The expression of TGF-β1m RNA,Smad3 m RNA and Smad7 m RNA in tumor tissues of each group was detected by RT-q PCR.Results:1.Serum indexes of tumorous nude mice: compared with blank group,the levels of alanine transaminase,aspartate transaminase and urea in each group were increased,while the level of creatinine was decreased(p < 0.05);Compared with model group,alanine transaminase(Alt),aspartate transaminase(Alt)and urea were decreased in all treatment groups,and the levels of creatinine were increased in combination group,with the most significant effect in combination group(p < 0.05).There was no statistical significance in the above four indexes between the bupleuron Vitex group and capecitabine group(p > 0.05).2.Tumor inhibition rate: compared with model group,tumor weight in bupleuron vitt group,capecitabine group and combination group was significantly decreased,with statistical significance(p < 0.05);The tumor weight of combination group was significantly decreased compared with that of chaihulongvitang group and capecitabine group,with statistical significance(p < 0.05).The inhibitory rates of bupleuron vitt group,capecitabine group and combination group were 28.26%,39.13% and 65.21%,respectively.3.HE staining results showed that,compared with the model group,the tumor tissue structure was destroyed and necrotic areas appeared in each treatment group.The apoptosis area of tumor tissue in combination group,capecitabine group and chaihulongvitang group increased significantly,and the necrotic area in combination group was the largest.4.Masson staining results showed that: compared with model group,fibrosis tissue area around tumor tissue was reduced in all treatment groups,and fibrosis degree of tumor tissue was significantly reduced.5.RT-q PCR results showed that compared with model group,TGF-β1 mrna and Smad3 m RNA expressions of nude mice in combination group,capecitabine group and bupleurongvitang group were decreased,while Smad7 m RNA expressions were increased(p < 0.05).In combination group,TGF-β1m RNA and Smad3 m RNA gene expressions were most downregulated,while Smad7 m RNA expression was most up-regulated(p <0.05).Conclusion:1.Chaihulongvitt combined with capecitabine can inhibit tumor growth in nude mice with triple negative breast cancer.2.Chaihulongvitt,capecitabine and the combination could inhibit the proliferation of tumor cells and improve the degree of fibrosis of tumor tissue,and the combination group had the most obvious effect.3.To clarify the mechanism of inhibiting triple-negative breast cancer by Chaihuulongvitt combined with capecitabine: the mechanism may promote tumor cell apoptosis by reducing the expression of TGF-β1 mrna and SMad3 mrna,increasing the expression of SMad7 mrna,and may affect triple-negative breast cancer by regulating TGF/Smad signaling pathway. |
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