| Based on the characteristic of near-infrared spectrum, analysis method and application in biomedicine, this dissertation mainly discuss on the followings: 1.Near-infrared spectrum measuring method and analysis of glucose powder; 2.Near-infrared spectrum measuring and analysis of glucose solution; 3.Near-infrared spectrum measuring system of blood sugar; 4.Near-infrared spectrum measuring and analysis of plasma and total blood; 5.Near-infrared spectrum system of urine sugar quantificational measurement, near-infrared spectrum measuring and analysis of urine sugar.This research shows: adopting near-infrared spectrum glucose can be measured quantificationally, new blood sugar measuring system is developed, which breaks through the traditional method and meets the state requirement of biochemistry inspection; adopting near-infrared spectrum urine sugar quantificational measurement is achieved, which provides a new approach for diabetes diagnosis.The development of near-infrared technology and its application on biomedicineThe near-infrared area refers to the electromagnetic wave whose wavelength range from 780 to 2526nm according to ASTM (American Society for Testing and Materials) which is the earliest discovered visible light with 200 years history. The near-infrared analysis technology develops in a devious process. With the development of computer and the naissance of Chemometries, the modern near-infrared analysis technology then occurs combined with the near-infrared spectrum. Especially the recent ten years, the near-infrared technology develops rapidly on instrumentation, software and application. Meanwhile its efficiency and speediness attract much public attention. These years near-infrared analysis technology is applied widely in life chemistry and medicine and it has become an evaluation tool of body composition. The conspicuous advantage of near-infrared spectrum is that the sample does not need any process preparation and no invasion and no hurt to the body. It can be used in measuring the change of skin tissue affected by environment, breast cancer detection, haemoglobin determination, clinic analysis, lipoprotein in serum analysis, body fluid analysis and blood oxygen measurement. The near-infrared spectrum can detect the body directly with no invasion. It makes the research work possible which in past cannot be imaged and enhances greatly the analyzing and detecting efficiency thus it plays an important role in life chemistry and clinic.Diabetes is a prevalent incretion disease at present. In America and Europe there are 3% of the population suffer the diabetes disease. In China with the development of living status, the incidence of diabetes is in a climbing trend. It is reported that by 2010, the number of diabetes sufferer will have reached 220,000,000. Usually detecting the blood sugar concentration and based on this to control the diet can help to postpone the diabetes and reduce the syndrome. At present patients usually are detected in the hospital or by themselves. The commonly adopted method is to put the biochemical reagent into serum, then using color comparison to determine the sugar concentration. This method needs reagent and a long measure process. So the suffer and doctor personnel need a fast exact detecting method. Detecting blood sugar by near-infrared spectrum is the important application on biomedicine. Many scholars and experts research on no invasion blood sugar detection, but there is no perfect application in clinic and home. So no invasion blood sugar detection has becoming a research hotspot nowadays.II．Routine diabetes detecting method To detect blood sugar, now the most commonly used method of is enzyme, including GOD,HK and GDH method. GOD-POD method makes use of the principle, that glucose is oxidated by GOD and produce H202, which is then catalysed by POD and is oxidated into colored chromogen from achromatous deoxidized chromogen. Thus the glucose content is determined by its shade of color. The original response of this method is di...
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