Isolation And Cloning Of Novel CDNAs Related To Human Brain Development And Differentiation

Human brain plays a crucial role in the whole course of an individual's life, from embryonic development to growth, maturation and aging. The development and differentiation of brain, which exerts a fundamental impact on brain's multiple functions, predominates as one of the most promising disciplines in biomedical sciences. It is genetically governed by a complex program of ordered and timely expression of a large variety of genes. Although the study of a series of genes including the homeotic family has yielded remarkable insight into the molecular mechanisms underlying the development of brain regarding both human and related model organisms, a great number of novel genes remain to be investigated. It will provide invaluable molecular information for better understanding of brain development and relevent neurological/psychological disorders to systematically isolate novel cDNAs involved in the development and differentiation of brain.Different human fetal brain tissues(cerebrum and cerebellum) and those at different developmental stage(13-week and 33-week) were subject to mRNA differential display analysis(also referred to as differential display RT-PCR or DDRT-PCR). Fifty-four combination of 16mer one-base anchored primer and 10mer arbitrary primer were utilized to implement DDRT-PCR. Approximately 150 PCR products of differential interest were displayed in sequencing gels. 85 PCR products of first priority , whose size varies between 100-500bp, were chosen for subsequent cloning and sequencing. 90 cDNA sequences were obtained , then submitted to and deposited in the dbEST division of GenBank under assigned BenBank...