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Detection Methods Of Vibrio Parahaemolyticus And Hepatitis A Virus And Bacterial Diversity Analysis In Sediments Along The Coasts

Posted on:2007-10-22Degree:DoctorType:Dissertation
Country:ChinaCandidate:J F FanFull Text:PDF
GTID:1100360185489311Subject:Biomass conversion and utilization project
Abstract/Summary:PDF Full Text Request
There are many pathogen microorganism in ocean, they can infect halobios and human. Marine pathogen microorganism pose a significant threat to seawater breed aquatics industry and human health, consequently marine pathogen microorganism monitoring have a important application spread potential for evaluating seafood safety and forecasting disease and lessening correlative economy loss.Recently, with the coastal city population increasing, pathogen with the life sewage pouring sea increased, Then, pathogen distributed in sea water, sediment and organisms, so they influence marine ecosystem stability by hurt halobios, and threaten human body health by contacting seawater or sediment and taking seafood. In 1988, hepatitis A crazed on account of eated shellfish. Pathogenic microorganism in sediment nearby sewage inlet can harm human body. Otherwise, disease have already become a bottle-neck of restricting marine breed aquatics industry development, Litopenaeus vannamei is one of the three important kinds of breeding shrimp in the world, recently often affected by red body disease. So it is indispensable to monitoring pathogenic microorganism.In china, methods to detecting marine pathogenic microorganism are still traditional not yet systemic and rapid, so as to routine monitoring is difficult. Therefore, it is indispensable to get methods which is systemic, special, simple; rapid and exact. In this paper, methods to detecting Vibrio parahaemolyticus which was the pathogen of the red body disease of Litopenaeus vannamei, hepatitis A viruses in seawater and shellfish, pathogenic microorganism in sediment nearby sewage inlet was studied and applied, and PCR-DGGE was applied for analysis of pathogenic microorganism diversity in sediment for the first time.In this study, pathogen of the red body disease of Litopenaeus vannamei was Vibrio parahaemolyticus by dominant bacteria separating, regressing infection assy, biochemical identification and 16S rRNA homology analysis. Three kinds of methods to detecting Vibrio parahaemolyticus were finished by use of Vibrio parahaemolyticus antigen and rabbit anti- Vibrio parahaemolyticus IgG, namely iELISA, Dot-ELISA, IFA. Three kinds methods were stabile and special; they had different sensitivity, IFA was highest and iELISA was higher and Dot-ELISA was lowests; they were all rapid, finished time were 2h, 9h and 7h respectively; they need different apparatus, Dot-ELISA is fittest for locally survey due to not requiring more apparatus. Otherwise, PCR for detecting Vibrio parahaemolyticus was developed on the basis of the primer designed by...
Keywords/Search Tags:Marine Pathogenic microorganism, Vibrio parahaemolyticus, Hepatitis A virus, bacterial diversity, Detection methods
PDF Full Text Request
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