Porcine Adipocyte Biology Characteristics And The Regulation Role Of Insulin & Cholesterol On Its Lipogenesis

Obesity is now a global epidemic and the prevalence of this important public health problem has increased dramatically in recent years. Because it is related with morbidity and mortality, the epidemic of obesity has raised concerns of researchers about its happening and development. The lipogenesis ability of pig is the highest in all of the livestock, and its physiology, anatomic and genomic traits are similar to human which may be favourable for used as the model animal in the field of obesity. On the other hand, typically economic, would be that of using genetic selection to drive metabolism towards the production of the so-called"functional foods"without regard for pig welfare or its ethical importance. The evidences suggesting that pig could be a better model for studying lipogenesis and its regulation.A primary goal of the obesity study is to understand the mechanism of adipose development and regulation. In fact, white adipose tissue expansion takes place rapidly as a result of the increased fat cell size as well as an increase in fat cells number, and the process is regulated by sterol regulatory element binding protein-1c (SREBP-1c) and other transcription factors. Primary culture adipocyte can often be used for studying the process. Nowadays, the culture system of porcine adipocyte is not well established yet in China. We established successfully the optimal system of primary porcine preadipocyte culture, and redisplayed the whole process of the proliferation and hyperprothy of porcine preadipocyte. To choose a better cryoprotectant for pig adipocyte, the effects of different kinds and concentrations cryoprotectants on the porcine preadipocyte of post-thawing survival rates, viability, proliferation and differentiation capacity were evaluated. On the basis of the primary porcine preadipocyte culture and cryopreservation, to explore the cell and molecular events during fat development, cells were exposed to insulin and cholesterol which were the inducement factors of SREBP-1c expression, the cell proliferatioin, differentiation, cellular TG content, lipogase activity were detected, furthermore, the transcription concentration of transcription factor SREBP-1c and enzymes involved in lipogenesis acetyl-CoA carboxylase (ACC), fatty acid synthetase (FAS), stearoyl-CoA desturase (SCD) were studied by RT-PCR. The results were as...