Experession Of Human Bone Morphogenitic Protein 2A, 3, And 1 In E. Coli.

During the latter part of the nineteenth century, Senn reported that decalcified bone could be used clinnically as a delivery system for antiseptics in the treatment of bone cavities . In the early 1960s, Urist and co-workers discovered that the consistent osteoinduction by decalcified bone can be achieved by control of time, temperature, and concentration of HC1. Urist and co-workers proposed that there is a bone formative inducer in decalicified bone. The inducer was a protein and named bone morphogenetic protein (BMP). In 1979 and also recently, Urist and co-workers have able to isolate BMP from demineralized rabbit, bovine, porcine, and human bone, teeth, and osteosarcoma. It was found that the osteoindutive potential of demineralized bone matrix was inhibited by pretreatment with heparinized plasma and heparin, possibly by binding to the active sites in the matrix. In view of this, Wang and co-workers have explored the utility of heparin affinity chomatography to purify bone-inductive protein. A factors that induces bone formation was purified 300,000-fold from guanidinium chloride extracts of demineralized bone by Wang and co-workers. The active component resied in a single gel band, crresponding to a molecular mass of 30KD, which yielded proteins of 30, 18, and 16KD on reduction. The attempt to further indentify the active BMP species by trying to separate the components of nonreduced 30KD material by various methods was unsuccessful. The partial amino acid sequence of 30KD material and 16KD protein was obtained. It was noted that BMP is very useful in treatment of...