Cloning And Function Analysis Of Apoptosis Related Genes For The Mandarin Fish Siniperca Chuatsi

The mandarin fish Siniperca chuatsi is an important species in China's aquaculture. The culture of the mandarin fish has been threatened seriously by many kinds of diseases. It is thus important and necessary to develop some effective methods for the control of diseases. The understanding of immune system in this fish will provide a base for such development. The present study aimed to clone some apoptosis-related genes for the mandarin fish, with their apoptotic function analyzed in cell lines.Three apoptosis related genes were cloned for the first time in the mandarin fish, i.e. TNF-related apoptosis-inducing ligand (TRAIL), a TRAIL decoy receptor (DcScTRAILR) and Mst3 and SOK1-related kinase (MASK) which are named as ScTRAIL, and ScDcTRAIL, and ScMASK. ScRAIL gene with the full-length cDNA of 1359 bp contains an open reading frame coding for a 283 aa protein; it's full length genome has 9021 bp which contains six exons and five introns. The promoter region of ScTRAIL is 732 bp, which contains some putative transcription factor binding sites such as Oct-1, Sp-1, NF-1, RAP-1, C/EBPalp, NF-kappaB and AP-1; however, it lacks typical TATA or CAAT box. The ScDcTRAILR gene with the full-length cDNA of 2494 bp contains an open reading frame coding for a 353 aa protein; its full-length genome sequence is 10437 bp which contains ten exons and nine introns. The promoter region of ScDcTRAILR is 804 bp, which contains some putative transcription factor binding sites such as C/EBPalp,Sp-1,Oct-1 and GATA-1, but it lacks...