| The Baculoviridae is a diverse family of arthropod-specific viruses with a double-stranded genomic DNA of which most members infect Lepidoptera. It has been widely utilized for the expression of recombinant proteins and as a biopesticide. With the development of biology, baculovirus became a new gene therapy vector. we have initiated experiments to determine whether baculovirus vectors are capable of delivering transgene to avian. One of our aims of this thesis is to analyze the efficiency of baculoviruses transducing into avian cells, to illuminate the response of avian cells stimulated by baculoviruses and the mode of entry of baculovirus into avian. We also expressed baculoviral p35 gene in kidney cells mediated by baculovirual vector and checked the suppression of apoptosis. We are very interested if expression of the baculovirus P35 protein in plant could enhance the broad-spectrum resistance against diseases. Our studies broadened the range of susceptible cells of baculovirus system and developed the studies of baculovirus as well as genes, which made a soild foundation for futher application research.In Chapter one, an overview outlines the character of baculovirus, major on the application of baculoviruse and the broad-spectrum anti-apoptotic gene p35.Baculovirus AcMNPV has shown the ability of high efficiently transducing into a variety of mammalian cell types. Chicken and duck cells were tested for their ability to be transduced by Ac-CMV-eGFP, a recombinant baculovirus capable of expressing an eGFP reporter gene under the control of the CMV promoter. Our results (Chapter 2) showed that chicken and duck cells were transducible, as demonstrated by flow cytometry assay, and the transduction efficiency of duck cells was higher than that of chicken cells. Addition of histone deacetylase inhibitor sodium butyrate enhanced the transduction of baculovirus into both chicken and duck cells. In addition,...
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