Studying Apamers Based On Fluorescence And The Construction Of Related Databases

Aptamers are a new class of synthetic DNA/RNA oligonucleotides generated from in vitro selection. They can selectively bind with various molecules. The emerging of aptamers changes the general view that biological effect of oligonucleotide is a function of the DNA/RNA codes involved in genetic transcription and translation. Due to aptamer self-folding into special tertiary structures for specific proteins and small drug molecules with high affinity (Kd≈nM or pM), more and more attentions have been paid to the applications of aptamers in detection, new drug development, medical diagnostics and so on. Now, it has been of increasing interest to develop the signaling aptamers with the capability of real-time detection of targets in a simple and rapid way. For its convenience, high sensitivity and selectivity, fluorescence spectrum has been one of the most popular detection strategies. One-labeling and double-labeling are the common ways. For example, oncoprotein PDGF-BB (platelet-derived growth factor BB) has been detected by capturing the change of the anisotropy or FRET resulting from targets. However, the labeling probability weakens the identification capacity, and casts much. It is especially difficult for labeling RNA aptamer. The fact that most aptamers from the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) are RNA blocks applications of aptamers in proteins detection and biosensors, which needs hardly analysis methods without labeling. However, there were few related reports.